摘要
Effective hydrolysis of feather waste by keratinase is an important way to develop high-quality alternative proteins for feed.It has been shown that the reduction of disulfide bonds is the key rate-limiting step in the hydrolysis of feathers by keratinases.However,the reductive activity of keratinase itself is low.In order to in-crease the reductive activity of the catalytic system,we screened endogenous disulfide reductase from Bacillus subtilis and constructed a synergistic catalytic system,KerZ1/yjbI-S2-P1,consisting of keratinase and disulfide reductase by co-expression optimization.The reductive activity and feather hydrolytic activity of the synergistic system were 5.67-fold and 5-fold higher than the original system.Feathers were hydrolyzed to a higher extent compared with the original system.The total amino acid and soluble protein concentrations in the products increased by 99%and 59%,respectively.This study provides a more environmentally friendly and efficient reduction capability for keratinase to hydrolysis waste feathers,and also demonstrates a new way of alternative protein feedstock mining.
基金
supported by the National Key Research and Devel-opment Program of China(2022YFD2101402)
the National Natural Science Foundation of China(22208125)
the Natural Science Foundation of Jiangsu Province(BK20221084).
