摘要
自枣树寄生粗毛纤孔菌(Inonotus hispidus)子实体组织分离获得菌株,采用三种培养基质(ZI1、ZI2、ZI3)栽培,测定6-(3,4-二羟基苯乙烯)-4-羟基-2-芘酮(hispidin)含量,并采用高效液相法测定子实体的指纹图谱。结果表明:最佳培养基质为ZI2(64%枣树木屑、16%棉籽壳、19%麦麸、1%生石灰),接种80~100 d后菌丝长满菌包,15~20 d后采收,出菇率为85%,产量为(12.87±1.53)g(干重);采用培养基质ZI2栽培子实体的Hispidin含量最高,为(141.70±1.20)μg·g^(-1);野生和3种培养基质栽培子实体的高效液相指纹图谱有8个共有峰(参照峰为Hispidin),相似度均大于0.99。
A strain was isolated from fruiting body tissue of Inonotus hispidus parasitizing on Ziziphus jujuba.The I.hispidus strain was then cultivated on three kinds of substrates(ZI1,ZI2,ZI3),and the resultant fruiting bodies were measured for hispidin[6-(3,4-dihydroxystyrl)-4-hydroxy-2-pyrone]content and analyzed for high-performance liquid chromatography(HPLC)fingerprint.The results showed that the optimal culture substrate ZI2(64%Z.jujuba wood chips,16%cottonseed hull,19%wheat bran,and 1%quicklime)allowed mycelia to fully colonize the cultivation bags within 80–100 d after inoculation,with fruiting bodies harvested 15–20 d thereafter.The fruiting rate of ZI2 reached 85%,and the yield was(12.87±1.53)g(dry weight).Among the three kinds of substrates,ZI2 also resulted in the highest hispidin content in fruiting bodies,reaching(141.70±1.20)μg·g^(-1).HPLC fingerprint analysis of the wild and the three groups of cultivated I.hispidus revealed eight common peaks(with hispidin as the reference peak),and the similarity indices were all greater than 0.99.
作者
王鑫
包海鹰
张广杰
李玉
WANG Xin;BAO Haiying;ZHANG Guangjie;LI Yu(Key Laboratory of Development and Utilization of Fungal Chinese Medicinal Resources,Jilin Agricultural University,Changchun 130000,Jilin,China;Key Laboratory of Edible Fungal Resources Utilization in Northern China,Ministry of Agriculture and Rural Affairs,Jilin Agricultural University,Changchun 130000,Jilin,China;Engineering Research Center of Edible and Medicinal Fungi,Jilin Agricultural University,Changchun 130000,Jilin,China)
出处
《食用菌学报》
北大核心
2026年第2期66-73,共8页
Acta Edulis Fungi
基金
吉林省桑黄资源开发利用跨区域合作科技创新中心
黄河故道桑黄的道地性及优质新品种研发(横20240180)。
关键词
枣树
粗毛纤孔菌
驯化
子实体
品质评价
Jujube tree
Inonotus hispidus
domestication
fruiting body
quality evaluation
