摘要
基于全基因组和转录组数据对艾Artemisia argyi NAC(AarNAC)基因家族成员进行鉴定,并分析其在不同组织及茉莉酸甲酯(MeJA)诱导后的表达模式,以期发现艾叶中次生代谢产物合成、植物生长发育及逆境胁迫响应调控相关的NAC转录因子。利用生物信息学方法在艾中共鉴定AarNAC基因家族成员176个,其编码的蛋白质由152~1269个氨基酸组成,相对分子质量为17.38~141.23 kDa,理论等电点为4.42~9.60。亚细胞定位显示,大部分AarNAC基因家族位于细胞核、细胞质,少部分位于内质网和叶绿体。根据拟南芥NAC家族分类,将AarNAC蛋白分为13个亚族。启动子顺式作用元件分析表明AarNAC家族与生长发育、激素响应和非生物胁迫密切相关。组织表达模式分析显示,AarNAC家族基因主要在根和茎中优势表达,且大部分基因受MeJA诱导表达,其中既在叶片中优势表达又受到MeJA诱导表达的AarNAC基因有25个,这些基因可能为艾叶中有效成分合成的关键调控基因。该研究为深入解析AarNAC家族基因的功能及其对艾叶药效成分积累的调控机制奠定了基础。
Based on whole-genome and transcriptome data,this study identified the gene members of Artemisia argyi NAC(AarNAC)family and analyzed their expression patterns in different tissues and methyl jasmonate(MeJA)-induced samples,aiming to discover NAC transcription factors related to secondary metabolite synthesis,plant growth and development,and stress response regulation in A.argyi.A total of 176 AarNAC genes were identified in A.argyi using bioinformatics methods.The encoded proteins were composed of 152-1269 amino acids.The relative molecular weights of AarNAC proteins were 17.38-141.23 kDa,and the theoretical isoelectric points were 4.42-9.60.Subcellular localization showed that most AarNAC gene family were located in the nucleus and cytoplasm,and a few were located in the endoplasmic reticulum and chloroplasts.According to the classification of Arabidopsis thaliana NAC gene family,AarNAC proteins were divided into 13 subfamilies.Analysis of promoter cis-acting elements showed that AarNAC family was closely related to growth and development,hormone response,and abiotic stress.Tissue expression pattern analysis showed that AarNAC family genes were mainly expressed in roots and stems,and most of the genes were induced by MeJA,among which 25 genes were predominantly expressed in leaves and also presented MeJA-induced expression.These AarNAC genes were presumed to be the key regulatory genes for the synthesis of active components in A.argyi.This study lays a foundation for in-depth analysis of the functions of AarNAC family genes and their regulatory mechanisms underlying the accumulation of bioactive compounds in A.argyi.
作者
肖闯
岳雪雪
刘大会
苗玉焕
XIAO Chuang;YUE Xue-xue;LIU Da-hui;MIAO Yu-huan(School of Pharmacy,HubeiUniversity of Chinese Medicine,Wuhan 430065,China;Hubei Shizhen Laboratory,Wuhan 430065,China)
出处
《中国中药杂志》
北大核心
2026年第5期1323-1333,共11页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(82304660)
湖北省中医药创新发展联合基金重点项目(2024AFD228)
湖北省自然科学基金创新群体项目(2023AFA032)
湖北中医药大学2024年度科技创新项目(2024KJCX005)。
关键词
艾
有效成分
NAC转录因子
生物信息学
表达模式
Artemisia argyi
active component
NAC transcription factor
bioinformatics
expression pattern
