摘要
为了探究氧化石墨烯(Graphene oxide, GO)对红树植物桐花树Aegiceras corniculatum胎萌苗生长与生理的影响,采用盆栽试验,使用0、10、20、50及100 mg·L^(-1)的GO浓度的GO溶液浇灌处理桐花树母树种子(胚轴),测定了桐花树胎萌苗的根系形态、植株生物量及根系与叶片的抗氧化酶活性等指标。结果表明:在培养60 d后, GO对桐花树胎萌苗根系生长呈现浓度依赖性双相效应,即20 mg·L^(-1)GO处理显著促进根系总长、根系表面积和生物量,较对照(不添加GO)分别增加56.2%、45.3%和39.2%;而当浓度≥50 mg·L^(-1)时则转为抑制。根系组织中超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性均随GO浓度升高呈整体下降趋势,其中100 mg·L^(-1) GO处理下SOD与POD活性分别较对照下降28.4%和42.3%。与之相反,50、100 mg·L^(-1) GO处理下叶片中POD和CAT活性较对照显著升高(P<0.05),表现出器官间的差异化应激响应。此外,各处理组根冠比均与对照差异不显著。研究表明, GO处理对桐花树胎萌苗产生一定程度的氧化胁迫,影响各项生理生化指标,20 mg·L^(-1) GO可能触发“生长-防御权衡”策略,促进根系形态建成;较高浓度(≥50 mg·L^(-1)GO),则产生显著抑制效应,影响桐花树胎萌苗生长。
To investigate the effects of graphene oxide(GO)on the growth and physiology of Aegiceras corniculatum seedlings,pot experiments were conducted using GO solutions with concentrations of 0,10,20,50,and 100 mg·L^(-1) to treat the seeds(hypocotyls)of A.corniculatum.The root morphology,plant biomass,and antioxidant enzyme activities in the roots and leaves of the seedlings were measured.The results showed that after 60 days of cultivation,GO exhibited a concentration-dependent biphasic effect on the root growth of A.corniculatum seedlings.Specifically,the 20 mg·L^(-1) GO treatment significantly promoted the total root length,root surface area,and biomass,increasing them by 56.2%,45.3%,and 39.2%,respectively,compared to the control(no GO).However,when the concentration reached or exceeded 50 mg·L^(-1),the treatment turned inhibitory.The activities of superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT)in root tissues all exhibited an overall downward trend with increasing GO concentration.Specifically,under 100 mg·L^(-1) GO treatment,SOD and POD activities decreased by 28.4%and 42.3%,respectively,compared to the control.Conversely,POD and CAT activities in leaves under 50 and 100 mg·L^(-1) GO treatments showed significant increases compared to the control(P<0.05),indicating differential stress responses among organs.Additionally,the root-shoot ratio in all treatment groups did not differ significantly from the control.The study demonstrates that GO treatment induces oxidative stress in seedlings of A.corniculatum,affecting various physiological and biochemical parameters.A concentration of 20 mg·L^(-1) GO may trigger a"growth-defense trade-off"strategy,promoting root morphogenesis.However,higher concentrations(≥50 mg·L^(-1) GO)exerted significant inhibitory effects,impairing the growth of seedlings.
作者
冯炜强
李林锋
伍世挺
李巧炫
廖雅敏
FENG Weiqiang;LI Linfeng;WU Shiting;LI Qiaoxuan;LIAO Yamin(Faculty of Chemistry and Environment Science,Guangdong Ocean University,Zhanjiang,Guangdong 524088,China)
出处
《林业与环境科学》
2026年第1期77-82,共6页
Forestry and Environmental Science
基金
广东省大学生创新创业研究项目(S20231056057)
广东省本科高校线上线下混合式一流课程建设项目(jxyj20231215017)。
关键词
桐花树
氧化石墨烯
根系生长
酶活性
Aegiceras corniculatum
graphene oxide
root development
enzyme activity
