摘要
旨在为富含油脂的餐厨垃圾的高效分解提供参考,从餐厨垃圾中筛选出高产脂肪酶的菌株,通过16SrDNA测序并构建发育树来鉴定菌株种属,并利用单因素实验和响应面实验优化产酶条件。结果表明:从餐厨垃圾中共分离出16株具有油脂降解能力的菌株,其中1株菌株的油脂降解率和脂肪酶酶活分别达到了76.61%和380.55U/mL,将其命名为CZU-JY13:分子学鉴定结果显示,该菌株属于空气芽孢杆菌(Bacillusaerius);菌株CZU-JY13的最优产酶条件为培养基初始pH 9.9、发酵温度37.0℃、培养基中NaCl质量浓度4.6g/L、接种量3%、发酵时间48h,在此条件下脂肪酶酶活高达(620.56±5.34)U/mL,较优化前提高63.07%。综上,菌株CZU-JY13是一种可以高效处理餐厨垃圾中油脂的菌种资源。
To provide a reference for the efficient decomposition of oily kitchen waste,a high lipase producing strain was screened from kitchen waste,identified via 16S rDNA sequencing and phylogenetic tree construction,and its enzyme production conditions were optimized using single-factor experiments and response surface methodology.The results showed that a total of 16 strains with oil degrading ability were isolated from kitchen waste,among which one strain exhibited a lipid degradation rate of 76.61%and a lipase activity of 380.55 U/mL.This strain was designated CZU-JY13.Molecular identification revealed that strain CZU-JY13 belonged to Bacillus aerius.The optimal enzyme production conditions for strain CZU JY13 were determined as follows:medium initial pH 9.9,fermentation temperature 37.0 C,NaCl mass concentration 4.6 g/L,inoculation volume 3%,and fermentation time 48 h.Under these optimized conditions,lipase activity reached(620.56±5.34)U/mL,representing a 63.07%increase compared to pre optimization levels.In conclusion,strain CZU-JY13 is a microbial resource with high potential for efficient degradation of oils in kitchen waste.
作者
邓婷婷
柳远哲
杨凌寒
张东慧
唐盛
王强
陈喜锋
梁剑光
DENG Tingting;LIU Yuanzhe;YANG Linghan;ZHANG Donghui;TANG Sheng;WANG Qiang;CHEN Xifeng;LIANG Jianguang(School of Pharmacy&School of Biological and Food Engineering,Changzhou University,Changzhou 213164,Jiangsu,China;Changzhou Yuankang Biotech Co.,Ltd.,Changzhou 213164,Jiangsu,China;Suzhou Yuankang Biotech Co.,Ltd.,Suzhou 215000,Jiangsu,China;Jiangsu Lvbo Biotech Co.,Ltd.,Changshu 215500,Jiangsu,China)
出处
《中国油脂》
北大核心
2026年第4期85-91,121,共8页
China Oils and Fats
基金
江苏省研究生科研实践创新计划(KYCX212866)。
关键词
餐厨垃圾
脂肪酶
油脂降解
空气芽孢杆菌
产酶工艺优化
响应面法
kitchen waste
lipase
oil degrading
Bacillus aerius
optimization of enzyme production process
response surface methodology
