摘要
种子萌发作为植物繁衍后代、开启新生命周期的关键环节,起始于成熟干燥种子的吸水膨胀,终止于胚根突破种皮。为探究种子萌发过程中胚的动态变化与种子萌发状态之间的关联,本研究以水稻种子‘日本晴’(‘Nipponbare’)为材料,提出一种基于显微计算机断层扫描(Micro-CT)与多模态分析的水稻种子萌发原位表征方法。在电压80 kV、电流100μA、单次曝光时间50 ms、扫描角度间隔0.5°的条件下,对萌发过程中特定时间点的水稻种子进行图像采集和3D重建,获取最终扫描图像并加以分析。从重建图像观察到,伴随吸水时间的延长,水稻种子的胚体积呈逐渐增大趋势,胚根突破种皮,随后胚芽开始生长,胚根长度由0 h的0 mm增长至48 h的2.3 mm,胚体积从0.12 mm^(3)增加至0.38 mm^(3)。本研究成功构建了基于Micro-CT技术的原位动态表征方法,为种子萌发的生理生化机制研究提供了原位可视化的分析手段,具有潜在应用价值。
Aims:Seed germination,as a crucial step in plant reproduction and the initiation of the new life cycle,begins with the water absorption and expansion of mature dry seeds,and ends with the emergence of the radicle through the seed coat.To investigate the correlation between the dynamic changes of the embryo during seed germination and the germination status,this study used'Nipponbare'rice seeds(Oryza sativa)as the material and proposed an in-situ characterization method for rice seed germination based on micro-computed tomography(Micro-CT)and multimodal analysis.Methods:Under the conditions of 80 kV voltage,100μA current,50 ms single exposure time,and 0.5°scanning angle interval,images of rice seeds at specific time points during germination were collected and 3D reconstructions were performed.Three key time points of the seeds were selected for analysis:0 h after water absorption(dry seed state),24 h after water absorption(water absorption process completed)and approximately 48 h after water absorption(when the seeds began to germinate).Using CT scanning technology and in combination with the Hiscan Reconstruct software,the microscopic two-dimensional structural images of rice seeds at different germination stages were obtained.Results:As the water absorption time increases,the embryo volume of rice seeds shows a gradually increasing trend.The radicle of the embryo breaks through the seed coat,and then the plumule begins to grow.The length of the radicle increases from 0 mm at 0 h to 2.3 mm at 48 h,and the embryo volume increases from 0.12 mm^(3)to 0.38 mm^(3).Conclusion:This study successfully developed an in-situ dynamic characterization method based on micro-computed tomography(Micro-CT)technology.This research provides an in-situ visual analysis tool for the study of the physiological and biochemical mechanisms of seed germination,and has potential application value.
作者
王凯鸿
李明骏
范婷婷
周婧
赵静
萧浪涛
王若仲
WANG Kaihong;LI Mingjun;FAN Tingting;ZHOU Jing;ZHAO Jing;XIAO Langtao;WANG Ruozhong(College of Bioscience and Biotechnology,Hunan Agricultural University,Hunan Provincial Key Laboratory of Phytohormones and Growth Development,Changsha 410128,China;Yuelushan Laboratory,Changsha 410128,China)
出处
《植物生理学报》
北大核心
2026年第2期232-240,共9页
Plant Physiology Journal
基金
国家自然科学基金(32261143733和31871714)。
关键词
种子萌发
显微CT扫描
3D重建
seed germination
micro-CT scanning
3D reconstruction
