摘要
本研究旨在探讨广叶绣球菌多糖(SLPs)对高脂饮食联合链脲佐菌素(STZ)诱导的糖代谢紊乱小鼠骨骼肌糖代谢功能的改善作用及分子机制。结果显示,SLPs干预显著减轻了小鼠的糖耐量受损和胰岛素抵抗,同时提高了骨骼肌糖原含量,并改善了骨骼肌的形态损伤。转录组分析共鉴定出965个差异表达基因(DEGs)。GO注释和富集分析表明这些基因主要参与组织发育、动物器官形态发生、小分子代谢过程、肌原纤维、糖原结合、有机酸结合等生物学过程。KEGG通路富集进一步揭示,DEGs显著富集于PI3k/Akt信号通路、糖尿病并发症相关的AGE-RAGE信号通路、胰岛素抵抗、AMPK信号通路等关键信号通路。此外,SLPs通过调控小鼠骨骼肌中AMPK/SIRT1/PGC-1α信号通路相关基因、蛋白的表达和关键酶的活性来改善骨骼肌的糖代谢紊乱,保护骨骼肌损伤,维持机体代谢稳态。本研究为SLPs作为功能食品原料的开发提供了重要的理论依据。
The study was designed to investigate the improvement effect and molecular mechanism of Sparassis latifolia polysaccharide(SLPs)on glucose metabolism in skeletal muscle of mice with glucose metabolism disorder induced by high-fat diet combined with streptozotocin(STZ).The results showed that SLPs intervention could significantly reduce the impaired glucose tolerance and insulin resistance and increase skeletal muscle glycogen content and improve the morphological damage of skeletal muscle caused by glucose metabolism disorder.Transcriptome analysis identified a total of 965 differentially expressed genes(DEGs).GO annotation and enrichment analysis showed that these genes were mainly involved in tissue development,animal organ morphogenesis,small molecule metabolism,myofibril,glycogen binding,and organic acid binding.The enrichment analysis of KEGG further revealed that DEGs were mainly enriched in PI3k/Akt signal pathway,AGE-RAGE signal pathway related to diabetic complications,insulin resistance,AMPK signal pathway and other signal pathways.In addition,SLPs could regulate the expression of genes and proteins related to AMPK/SIRT1/PGC-1α signal pathway and the activities of key enzymes in skeletal muscle of mice to improve glucose metabolism disorder in skeletal muscle,protect skeletal muscle damage,and maintain the metabolic homeostasis of the body.This study provides a theoretical basis for the development of SLPs as functional food ingredients.
作者
王振
靳雯
魏欣
曹谨玲
云少君
程菲儿
冯翠萍
WANG Zhen;JIN Wen;WEI Xin;CAO Jinling;YUN Shaojun;CHENG Feier;FENG Cuiping(Shanxi Institute for Functional Food,Shanxi Agricultural University,Taiyuan 030031,Shanxi,China;College of Food Science and Engineering,Shanxi Agricultural University,Jinzhong 030801,Shanxi,China)
出处
《菌物学报》
北大核心
2026年第3期106-123,共18页
Mycosystema
基金
山西省科技成果转化引导专项项目(202304021301055)
山西省现代农业产业技术体系建设专项资金(2025CYJSTX09-02)
山西省科技重大专项计划(202301140601015)
山西省专利转化专项计划项目(202301009)。
