摘要
暗褐脉柄牛肝菌Phlebopus portentosus是牛肝菌中首个实现人工驯化栽培的物种,近年来其产业化栽培技术逐步成熟,规模持续扩大,因此其品种精准鉴定与保护至关重要。本研究对66株不同产地的暗褐脉柄牛肝菌菌株进行全基因组重测序,构建了包含306个位点的多核苷酸多态性(MNP)分子标记数据库,基于单核苷酸多态性SNP和MNP数据的群体结构与亲缘关系分析显示供试菌株可划分为2个主要群体。继代培养不同代数菌株的数据表明MNP标记具有良好的稳定性和分辨力,能实现菌株间的精准鉴定。通过对同一菌株不同批次的重测序数据分析,建立了克隆菌株判定标准(血缘同源性PI_HAT>0.95且遗传距离D<0.01);当遗传相似度(GS)达到100%时,可将两样本判定为同一菌株。结合机器学习进一步筛选出对群体分化具有指示意义的关键标记位点,为品种鉴定和功能基因研究奠定基础。本研究构建了暗褐脉柄牛肝菌分子标记数据库,验证了其在遗传资源保护与分子育种中的应用潜力,为品种权保护及种质创新提供科技支撑。
Phlebopus portentosus,the first successfully domesticated and cultivated bolete species,has undergone rapid industrial expansion in recent years,highlighting the growing need for accurate strain identification and germplasm protection.In this study,whole-genome resequencing was performed on 66 strains of P.portentosus collected from various regions,resulting in the construction of a database of multiple nucleotide polymorphism(MNP)markers containing 306 loci.Population structure and kinship analyses based on both single nucleotide polymorphisms(SNPs)and MNPs consistently grouped the strains into two distinct genetic clusters.The MNP markers exhibited high resolution and stability across successive subculturing generations,enabling precise strain discrimination.Clonal identification criteria were established using replicate sequencing data of the same strains,with thresholds set at PI_HAT>0.95 and genetic distance(D)<0.01,while a genetic similarity(GS)value of 100% within the MNP dataset was validated as a robust indicator of strain identity.Additionally,machine learning approaches were employed to identify key MNP loci indicative of population divergence,offering valuable markers for varietal authentication and functional genomics.This work established MNP molecular marker database for P.portentosus and validated the potential applications in genetic resource conservation and molecular breeding,providing scientific support for variety protection and germplasm innovation.
作者
孟国良
李嘉嘉
曹瑶
李凡
曾雨康
VLASENKO Vyacheslav Alexandrovich
李荣春
董彩虹
MENG Guoliang;LI Jiajia;CAO Yao;LI Fan;ZENG Yukang;VLASENKO Vyacheslav Alexandrovich;LI Rongchun;DONG Caihong(State Key Laboratory of Microbial Diversity and Innovative Utilization,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;Yunnan Junshijie Biotechnology Co.Ltd.,Yunnan(Rare Edible Fungi)Enterprise Technology Center,Kunming(Edible Fungi)Enterprise Technology Center,Kunming 650200,Yunnan,China;Institute of Edible Fungi,Yunnan Agricultural University,Kunming 650201,Yunnan,China;Central Siberian Botanical Garden of the Siberian Branch of the Russian Academy of Sciences,Novosibirsk 630090,Russia)
出处
《菌物学报》
北大核心
2026年第3期60-77,共18页
Mycosystema
基金
云南省(珍稀食用菌)企业技术中心专项资金(JSJ2023016KJ)。
关键词
暗褐脉柄牛肝菌
群体结构
多核苷酸多态性
遗传相似度
菌株鉴定
Phlebopus portentosus
population structure
multiple nucleotide polymorphism
genetic similarity
strain identification
