摘要
为探究不同耕作方式对植烟土壤微生物群落的影响,采用高通量测序技术对轮作、连作土壤中的微生物进行测定。结果表明:α多样性指数显示轮作土壤微生物多样性高于连作土壤;PCoA分析证实,连作与轮作土壤细菌群落结构存在显著差异;在门水平上,细菌变形菌门(Proteobacteria)和真菌子囊菌门(Ascomycota)均为轮作和连作土壤中的优势菌门;在属水平上,厌氧菌属(Anaeromyxobacter)和耐碱酵母属(Galactomyces)在轮作和连作土壤中富集;布丝酵母属(Boothiomyces)可作为连作土壤中的指示微生物;青霉属(Penicillium)为轮作土壤中的指示微生物。此外,速效钾(AK)和碱解氮(AN)分别是这2种土壤中细菌和真菌群落的主要影响因素。总的来说,轮作能提高土壤微生物多样性。
This study aims to investigate the effect of various agricultural practices on the microbial community composition in tobacco soil.Soil samples were collected from plots under rotational and continuous tobacco cultivation,and microbial communities were analyzed by high-throughput sequencing.Theα-diversity indexes showed that the microbial diversity in the soil of rotational cultivation was higher than that in the soil of continuous cultivation.Principal coordinate analysis clear distinguished the bacterial community structures between the two cultivation systems.At the phylum level,Proteobacteria and Ascomycota were dominant in the soil samples of rotational and continuous cultivation.At the genus level,Anaeromyxobacter and Galactomyces were more abundant.Notably,Boothiomyces was identified as the potential biomarker for continuous cultivation,whereas Penicillium was identified as that for rotational cultivation.Furthermore,available potassium and alkali-hydrolyzable nitrogen were the main influencing factors for the bacterial and fungal communities,respectively,in the soil of the two cultivation systems.These results indicated that implementing rotational cultivation could enhance the microbial diversity in the soil.
作者
周喜新
赵杨
孙敬钊
易丽君
胡伟
粟桂蓉
杨磊
ZHOU Xi-xin;ZHAO Yang;SUN Jing-zhao;YI Li-jun;HU Wei;SU Gui-rong;YANG Lei(College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128,PRC;Changde Branch of Hunan Tobacco Company,Changde 415000,PRC;Pu'er Branch of Yunnan Tobacco Company,Pu'er 665000,PRC;China Tobacco Hunan Industrial Co.,Ltd.,Changsha 410019,PRC)
出处
《湖南农业科学》
2026年第2期36-44,共9页
Hunan Agricultural Sciences
基金
湖南中烟工业有限责任公司科技项目(KY2025JD0004)。
关键词
轮作
连作
土壤微生物
多样性
群落结构
rotational cultivation
continuous cultivation
soil microorganisms
diversity
community structure
