摘要
Total antioxidant capacity(TAC)quantification is pivotal for biomedical diagnostics and food quality control.Herein,we developed a smartphone-integrated colorimetric platform leveraging a Fe-Mn dual-single-atom nanozyme(Fe/Mn-N-C)with enhanced peroxidase-like activity.Density functional theory calculations revealed that the unique diatomic synergy reduced the energy barrier during the catalytic process,thus endowing Fe/MnN-C with superior peroxidase-like activity.The inhibition mechanism of different types of antioxidants on the color reaction was investigated.The system quantified AA via antioxidant-induced suppression of oxTMB formation and applied successfully to the TAC detection of several fruit juices and commercial beverages.This work provided a new perspective for designing advanced single-atom nanozyme and developed a low-cost and easy-to operate TAC detection strategy with good sensitivity and specificity.
基金
the financial support from the“New Universities 20”Foundation of Jinan(Grant No 2021GXRCO99,T202204)。
