摘要
盐害是制约柑橘产量和品质提升的主要非生物胁迫之一。为了研究木霉与柑橘互作应答盐胁迫的机制,以柑橘砧木枸头橙(Citrusaurantium L.‘Goutoucheng’)为试验材料,研究正常条件和盐胁迫下接种哈茨木霉(Trichodermaharziauum)对柑橘种子萌发和幼苗生长生理的影响。结果表明,正常条件下接种木霉能促进种子萌发和幼苗生长,接种哈茨木霉的种子萌芽率,幼苗株高、茎粗、总根长、根表面积、根体积和干鲜质量等都显著高于空白对照。盐胁迫下,种子萌芽率和幼苗生长指标均显著降低,接种木霉能够有效缓解50mmol/L盐胁迫对种子萌芽率和幼苗生长的抑制。50mmol/L盐胁迫下,接种哈茨木霉后幼苗的株高、茎粗、地上部干鲜质量、地下部干鲜质量、总根长、根系投影面积、根表面积、根体积、叶片可溶性糖、叶片可溶性蛋白和叶片脯氨酸显著提高,提高叶片超氧化物歧化酶、过氧化物酶、氧化氢酶活性,降低丙二醛含量。接种哈茨木霉能显著缓解50mmol/L盐胁迫对种子萌发和幼苗生长的抑制作用。
Salinity is one of the main abiotic stresses severely affecting citrus yield and quality.To reveal the mechanism underlying the interaction between Trichoderma and citrus in response to salt stress,Citrus aurantium L.‘Goutoucheng’sour orange was used as the experimental material.The effects of T.harziauum on citrus seed germination and seedling growth were investigated under both normal and salt stress conditions.The results showed that Trichoderma inoculation could improve seed germination rate and seedling growth under normal conditions.The germination rate,plant height,stem diameter,total root length,root surface area,root volume,and fresh and dry weights of inoculated seedlings were significantly higher than those of the blank control.Under salt stress,seed germination rate and seedling growth were significantly reduced,while inoculation with Trichoderma could effectively alleviate the inhibitory effects of 50 mmol/L salt stress on seed germination rate and seedling growth.Under 50 mmol/L salt stress,the plant height,stem diameter,aboveground fresh and dry weights,underground fresh and dry weights,total root length,root projected area,root surface area,root volume,leaf soluble sugar,leaf soluble protein,and leaf proline content of the seedlings inoculated with T.harziauum were significantly higher than those of the uninoculated seedles.Additionally,it enhanced the activities of leaf superoxide dismutase,peroxidase,and catalase,while reduced the content of malondialdehyde.Inoculation with T.harziauum could significantly alleviate the inhibitory effects of 50 mmol/L salt stress on seed germination and seedling growth.
作者
赵美兰
金龙飞
岳杨
王文静
张德健
王鹏
Zhao Meilan;Jin Longfei;Yue Yang;Wang Wenjing;Zhang Dejian;Wang Peng(College of Horticulture and Gardening,Yangtze University,Jingzhou,Hubei,434025,China;Institute of Citrus Research,Zhejiang Academy of Agricultural Sciences,Taizhou,Zhejiang,318026,China)
出处
《中国南方果树》
北大核心
2026年第1期48-53,59,共7页
South China Fruits
基金
浙江省基础公益研究计划项目(LTGN23C150001)
黄岩区院地合作项目(黄岩蜜橘优质轻简栽培技术研究与示范)资助。
关键词
木霉
盐胁迫
柑橘
种子萌发
抗氧化酶
Trichoderma
salt stress
citrus
seed germination
antioxidant enzymes
