摘要
【目的】探明大花红山茶花粉形态特征及不同外源物质对大花红山茶花粉萌发特性的影响。【方法】以4个大花红山茶优系(HHCM-6、HHCM-7、HHCM-8、HHCM-9)花粉为材料,采用扫描电子显微镜观察花粉形态,运用固体离体培养法研究不同质量浓度的硼酸(boric acid, H_(3)BO_(3))、萘乙酸(1-naphthaleneacetic acid, NAA)和赤霉素(gibberellic acid, GA_(3))单因素对大花红山茶花粉离体萌发的影响,并采用正交试验优化大花红山茶花粉的萌发条件。【结果】(1)大花红山茶花粉呈长球形,极面观为三裂近圆形,赤道面观为长球形,极轴长57.59~69.17μm,花粉较大;外壁纹饰为皱波状(HHCM-6、 HHCM-8和HHCM-9)和穴网状(HHCM-7)。(2)适宜质量浓度的H_(3)BO_(3)、NAA和GA_(3)均能促进花粉萌发与花粉管伸长,50~200 mg·L^(-1) H_(3)BO_(3)、0.05~0.50 mg·L^(-1) NAA、0.5~5.0mg·L^(-1) GA_(3)能明显促进花粉萌发和花粉管伸长。(3)正交试验结果显示不同大花红山茶优系花粉离体萌发的最佳外源物质配比不同。【结论】4个大花红山茶优系花粉形态存在一定相似性;促进HHCM-6、HHCM-7花粉离体萌发最佳外源物质配比为100 mg·L^(-1) H_(3)BO_(3)+0.05 mg·L^(-1) NAA+1.5 mg·L^(-1) GA_(3),HHCM-8为200 mg·L^(-1) H_(3)BO_(3)+0.05 mg·L^(-1)NAA+2.5 mg·L^(-1) GA_(3),HHCM-9为100 mg·L^(-1) H_(3)BO_(3)+0.5 mg·L^(-1) NAA+0.5 mg·L^(-1) GA_(3)。研究结果可为大花红山茶人工辅助授粉、促进大花红山茶花粉萌发的营养液研究及杂交育种实践提供参考依据。
【Objective】Additives in culture medium to promote germination of Camellia magniflora pollens were studied.【Method】Pollens were collected from 4 elite lines of C.magniflora for morphological observation under a scanning electron microscope(SEM).In vitro culture medium was supplemented with a combination of H_(3)BO_(3),NAA,and GA_(3) in varied concentrations to determine the effects of the addition on pollen germination.Orthogonal experiments were conducted to optimize the formulation.【Results】(1)In a polar view,C.magniflora pollens were triangular-lobed and suborbicular,and in equatorial view,prolate with relatively large grains of the polar axis ranging from 57.59μm to 69.17μm long.The pollens of HHCM-6,HHCM-8,and HHCM-9 had a rugulate exine ornamentation,while those of HHCM-7 were foveolate-reticulate.(2)Although the additions of H_(3)BO_(3),NAA,and GA_(3) in the test ranges of concentration could all stimulate germination and elongate tubes of the pollens,the combination of 50−200 mg·L^(-1) H_(3)BO_(3),0.05−0.50 mg·L^(-1) NAA,and 0.5−5.0 mg·L^(-1) GA_(3) significantly enhanced them.(3)The optimal formulation of these additives varied for the different lines of C.magniflora.【Conclusion】The pollens of the 4 superior lines of C.magniflora were similar morphologically.The optimal combinations of the additives were 100 mg·L^(-1) H_(3)BO_(3)+0.05 mg·L^(-1) NAA+1.5 mg·L^(-1) GA_(3) for the pollens of HHCM-6 and HHCM-7,200 mg·L^(-1) H_(3)BO_(3)+0.05 mg·L^(-1) NAA+2.5 mg·L^(-1) GA_(3) for those of HHCM-8,and 100 mg·L^(-1) H_(3)BO_(3)+0.5 mg·L^(-1) NAA+0.5 mg·L^(-1) GA_(3) for those of HHCM-8.
作者
朱周俊
杨燃
许斌
周文君
吴泽龙
吴静逸
赵君茹
高晓敏
ZHU Zhoujun;YANG Ran;XU Bin;ZHOU Wenjun;WU Zelong;WU Jingyi;ZHAO Junru;GAO Xiaomin(Engineered Research Center for Innovation and Utilization of Oil Camelia Germplasm Resources,Guizhou Provincial Education Department/Guizhou Provincial Key Laboratory for Biodiversity Conservation and Utilization in Fanjing Mountain Region/College of Agriculture and Forestry Engineering and Planning,Tongren University,Tongren,Guizhou 554300,China;Huaihua Institute of Forestry Science,Huaihua,Hunan 418000,China;Hunan Botanical Garden,Changsha,Hunan 410116,China)
出处
《福建农业学报》
北大核心
2026年第1期52-61,共10页
Fujian Journal of Agricultural Sciences
基金
贵州省教育厅青年人才成长项目(黔教技[2024]223号)
铜仁市科学技术局博士人才项目(铜市科研[2024]2号)。
关键词
大花红山茶
花粉
硼酸
萘乙酸
赤霉素
Camellia magniflora
pollen
boric acid
1-naphthaleneacetic acid
gibberellic acid
