摘要
枇杷蜂蜜是重庆三峡库区的特色商品蜜种,本文通过测定总酚、总黄酮含量和评价抗氧化、抑菌活性,与市售的其他4种枇杷蜂蜜对比,并探究重庆三峡枇杷蜂蜜的益生活性。运用DPPH自由基、ABTS^(+)自由基清除能力评价蜂蜜的抗氧化能力,将最小抑菌浓度和抑菌率用于表征蜂蜜对革兰氏阳性菌(金黄色葡萄球菌)、革兰氏阴性菌(大肠杆菌)的抑菌活性。采用体外共培养结合生长曲线测定方法探究不同浓度的重庆三峡库区枇杷蜂蜜对保加利亚乳杆菌、青春双歧杆菌和嗜热链球菌的生长促进作用。结果表明,重庆三峡库区枇杷蜂蜜及其他枇杷蜂蜜样品均表现出不同程度的抗氧化和抑菌活性。重庆三峡库区枇杷蜂蜜的总酚和总黄酮含量分别为51.63±1.09 mg GAE/100 g、22.93±1.09 mg RE/100 g,均高于市售其他样品。在抗氧化能力方面,蜂蜜样品DPPH自由基清除率IC_(50)、ABTS^(+)自由基清除率IC_(50)均与总酚和总黄酮含量呈极显著负相关(P<0.01)。5种枇杷花蜂蜜样品均对金黄色葡萄球菌和大肠杆菌有抑制效果,其中重庆三峡库区枇杷花蜂蜜表现出最强的抑菌效果,对金黄色葡萄球菌和大肠杆菌的最低抑菌浓度分别为128 mg/mL和256 mg/mL。在益生活性方面,重庆三峡库区枇杷花蜂蜜在0~8、0~10、0~6 mg/mL的浓度区间下分别对保加利亚乳杆菌、嗜热链球菌和青春双歧杆菌的生长表现出促进作用,其最大比生长速率和生物总量分别出现在6、0、6 mg/mL及8、10、6 mg/mL时,表明其具有潜在益生元特性。上述结果表明,重庆三峡库区枇杷蜂蜜具有较高总酚、总黄酮含量、较强的抗氧化、抑菌活性和潜在的益生活性,品质较好。本研究为重庆三峡库区枇杷蜂蜜的抗氧化、抑菌和益生活性及其物质基础提供了基础科学数据,为其资源的进一步挖掘和功能产品的开发奠定了基础。
Loquat honey is a characteristic commercial honey variety from the Chongqing Three Gorges Reservoir Area.This study measured the total phenolic and total flavonoid contents,evaluated the antioxidant and bacteriostatic activities of this honey,and compared it with four other commercially available loquat honeys.The probiotic activity of Chongqing Three Gorges loquat honey was also investigated.The antioxidant capacity was assessed using DPPH and ABTS^(+)radical scavenging assays.The minimum inhibitory concentration(MIC)and inhibition rate were used to characterize the bacteriostatic activity against Gram-positive bacteria(Staphylococcus aureus)and Gram-negative bacteria(Escherichia coli).An in vitro co-culture method combined with growth curve analysis was employed to investigate the growthpromoting effects of different concentrations of Chongqing Three Gorges Reservoir Area loquat honey on Lactobacillus bulgaricus,Bifidobacterium adolescentis,and Streptococcus thermophilus.The results indicated that loquat honey from the Chongqing Three Gorges Reservoir Area and other commercially available samples exhibited varying degrees of antioxidant and bacteriostatic activity.The total phenolic and total flavonoid contents of Chongqing Three Gorges Reservoir Area loquat honey were 51.63±1.09 mg/100 g(gallic acid equivalent)and 22.93±1.09 mg/100 g(rutin equivalent),respectively,both higher than those of commercially available samples.Regarding antioxidant capacity,the IC_(50) values for DPPH and ABTS^(+)radical scavenging of the honey samples were negatively correlated with both total phenolic and total flavonoid contents.All five loquat honey samples inhibited Staphylococcus aureus and Escherichia coli,with Chongqing Three Gorges Reservoir Area loquat honey exhibiting the strongest antibacterial effect,showing MICs of 128 mg/mL and 256 mg/mL against S.aureus and E.coli,respectively.Regarding probiotic activity,Chongqing Three Gorges Reservoir Area loquat honey promoted the growth of L.bulgaricus,S.thermophilus,and B.adolescentis within the concentration ranges of 0~8,0~10,and 0~6 mg/mL,respectively.The maximum specific growth rates and maximum biomass were observed at 6,0,and 6 mg/mL,and at 8,10,and 6 mg/mL for the three bacteria,respectively,indicating its potential prebiotic properties.These results demonstrate that Chongqing Three Gorges Reservoir Area loquat honey possesses high total phenolic and flavonoid contents,strong antioxidant and antibacterial activities,and potential probiotic activity,indicating its superior quality.This study provides fundamental scientific data and theoretical basis on the antioxidant,antibacterial,and probiotic activities of Chongqing Three Gorges Reservoir Area loquat honey and their material basis,laying a foundation for further resource utilization and the development of functional products.
作者
龙道崎
万国超
甘芳瑗
庞钶靖
杨思静
杨桦
姜容
刘振平
LONG Daoqi;WAN Guochao;GAN Fangyuan;PANG Kejing;YANG Sijing;YANG Hua;JIANG Rong;LIU Zhenping(Chongqing Vocational Institute of Safety Technology,Chongqing 404000,China;School of Food and Bioengineering,Xihua University,Chengdu 610039,China;Chongqing Key Laboratory of Speciality Food Co-built by Sichuan and Chongqing,Chengdu 610039,China)
出处
《食品工业科技》
北大核心
2026年第7期31-40,共10页
Science and Technology of Food Industry
基金
重庆市万州区科研项目(wzstc-20230216)
重庆市教育委员会科学技术研究项目(KJQN202404708)。
关键词
重庆三峡库区
枇杷蜂蜜
功能成分
生物活性
益生活性
Chongqing Three Gorges Reservoir Area
loquat honey
functional components
biological activity
prebiotic activities
