摘要
培养基中激素和大量元素等是植物离体快繁的重要影响因素,为了探究激素和大量元素对紫萁离体快繁的影响,试验以紫萁成熟孢子为外植体,对孢子萌发、配子体增殖、孢子体诱导,以及生根等阶段的培养基配方进行了筛选。结果表明,紫萁孢子在1/8MS培养基中萌发效果较好,35 d配子体可发育成熟;配子体在1/2MS+1.5 mg/L 6-BA+0.5 mg/L NAA培养基中增殖效果最佳,增殖倍数可达4.63倍。在诱导孢子体阶段,大量元素中的NH_(4)NO_(3)对配子体形成孢子体起主要作用,而KH_(2)PO_(4)对孢子体的高生长有促进作用,大量元素的最佳组合为950 mg/L KNO_(3)+206 mg/L NH_(4)NO_(3)+185 mg/L MgSO_(4)·7H_(2)O+170 mg/L KH_(2)PO_(4)+110 mg/L CaCl_(2)·2H_(2)O;生根配方则以1/2MS+0.1 mg/L IBA+0.3 g/L活性炭为佳。本研究建立了紫萁离体快繁技术体系,为其组培工厂化育苗提供了一定的理论基础和技术支撑。
Medium components and hormones are important evaluation factors for rapid in vitro propagation of plants.In order to explore their effects on the rapid propagation of Osmunda japonica in vitro,the formulas of spore germination,gametophyte proliferation,sporophyte induction and rooting were screened with the mature spores of O.japonica as materials.The results show that O.japonica spores germinated best on 1/8MS medium,and the gametophyte developed and matured in 35 days;the formula suitable for gametophyte proliferation is 1/2 MS+1.5 mg/L 6-BA+0.5 mg/L NAA and the multiplication ratio was 4.63 times.In the sporophyte induction stage,NH_(4)NO_(3)promoted proliferation of sporophyte,KH_(2)PO_(4)promoted the high growth of sporophyte,950 mg/L KNO_(3)+206 mg/L NH_(4)NO_(3)+185 mg/L MgSO_(4)·7H_(2)O+170 mg/L KH_(2)PO_(4)+110 mg/L CaCl_(2)·2H_(2)O were suitable for sporophyte induction.The best rooting formula is 1/2MS+0.1 mg/L IBA+0.3 g/L activated carbon.In this study,the rapid in vitro propagation technology system of O.japonica was established successfully,theoretical basis and technical support for tissue culture and industrialized seedling raising of O.japonica was provided.
作者
孙大宽
唐军荣
王婷
汪海龙
田自能
常健红
向建英
Sun Dakuan;Tang Junrong;Wang Ting;Wang Hailong;Tian Zineng;Chang Jianhong;Xiang Jianying(Yunnan Academy of Biodiversity,Southwest Forestry University,Kunming,650224;Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China,Southwest Forestry University,Kunming,650224)
出处
《分子植物育种》
北大核心
2026年第3期818-827,共10页
Molecular Plant Breeding
基金
国家自然科学基金项目(31760086)
云南省“三区”科技人才支持计划(902100994)共同资助。
