摘要
目的:探索美洲大蠊提取物CⅡ-3对环磷酰胺诱导的肠道免疫损伤的影响。方法:将40只Balb/c小鼠随机分为正常组、模型组及CⅡ-3低、中、高剂量组(25、50、100 mg/kg)。除正常组外,其余各组连续3 d腹腔注射40 mg/kg环磷酰胺建立肠道免疫损伤模型,自第4天起,各给药组灌胃相应剂量CⅡ-3,正常组和模型组给予0.9%氯化钠溶液,持续14 d。检测粪便含水率、派伊尔结(PPs)数量及肠道IgA、IgM、sIgA含量;通过苏木精-伊红染色观察组织形态,实时荧光定量PCR检测相关基因mRNA表达水平。结果:环磷酰胺显著降低粪便含水率、PPs数量及IgA、IgM、sIgA水平,并造成肠黏膜结构破坏。CⅡ-3处理可剂量依赖性提高粪便含水率及IgA、IgM、sIgA水平,增加PPs数量并改善组织损伤;CⅡ-3中、高剂量组可上调T-bet、GATA-3及Foxp3表达,下调RORγt,恢复Th1/Th2与Th17/Treg平衡;CⅡ-3高剂量组显著上调Occludin、Claudin-1和Zo-1表达,改善肠道物理屏障功能。结论:CⅡ-3可通过调节肠道免疫细胞平衡、促进抗体生成及增强紧密连接蛋白表达,改善环磷酰胺诱导的肠道损伤并增强黏膜免疫功能,为其作为肠道免疫损伤干预材料提供理论依据。
Objective:To investigate the effects of Periplaneta americana extract CⅡ-3 on cyclophosphamide-induced intestinal immune injury.Methods:Forty Balb/c mice were randomly divided into a normal group,a model group,and low-,medium-,and high-dose CⅡ-3 groups(25,50,and 100 mg/kg).Except for the normal group,all mice received intraperitoneal injections of cyclophosphamide(40 mg/kg)for three consecutive days to establish the model.From day 4,mice in the treatment groups were administered CⅡ-3 by gavage for 14 days,while the normal and model groups received 0.9%sodium chloride solution.Fecal water content,Peyer′s patches(PPs)numbers,and intestinal IgA,IgM,and sIgA levels were measured.Histopathological changes were evaluated by hematoxylin-eosin staining,and mRNA expression of immune-related genes was detected by real-time quantitative PCR.Results:Cyclophosphamide significantly reduced fecal water content,PPs numbers,and IgA,IgM,and sIgA levels,and caused intestinal mucosal damage.CⅡ-3 treatment dose-dependently increased fecal water content and immunoglobulin levels,elevated PPs numbers,and alleviated histological injury.Medium-and high-dose CⅡ-3 upregulated T-bet,GATA-3,and Foxp3 expression while downregulating RORγt,thereby restoring Th1/Th2 and Th17/Treg balance.High-dose CⅡ-3 significantly increased the expression of Occludin,Claudin-1,and Zo-1,improving intestinal barrier function.Conclusion:CⅡ-3 ameliorates cyclophosphamide-induced intestinal injury and enhances mucosal immune function by regulating immune cell balance,promoting antibody production,and enhancing tight junction protein expression,providing a theoretical basis for its application in intestinal immune injury intervention.
作者
闫娜
吴广俊
张维炜
刘晓波
郭美仙
Yan Na;Wu Guangjun;Zhang Weiwei;Liu Xiaobo;Guo Meixian(College of Pharmacy,Dali University,Dali,Yunnan 671000,China;Sanmen County People′s Hospital,Taizhou,Zhejiang 317100,China)
出处
《大理大学学报》
2026年第2期15-21,共7页
Journal of Dali University
基金
云南省地方本科高校基础研究联合专项资金项目(202101BA070001-121,202101BA070001-119)
云南省兴滇英才支持计划团队专项(202305AS350001)
云南省昆虫生物医药研发重点实验室开放课题(AT2024002)。
关键词
美洲大蠊
CⅡ-3
环磷酰胺
肠道免疫
紧密连接蛋白
Periplaneta americana
CⅡ-3
cyclophosphamide
intestinal immunity
tight junction protein
