摘要
目的通过网络药理学方法探讨补中益气汤干预肌少症的潜在作用机制。方法基于中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)筛选补中益气汤中7味药材的活性成分;借助TCMSP及瑞士靶点预测数据库获得活性成分靶点;从疾病数据库中提取肌少症相关疾病靶点,获取中药复方活性成分相关靶点及疾病靶点的交集,进行蛋白–蛋白互作网络分析,开展基因本体功能及京都基因与基因组百科全书通路富集分析。最后进行核心成分与关键靶点的分子对接验证。结果共检索到补中益气汤182个活性成分、1139个成分靶点,肌少症相关的疾病靶点415个,交集靶点62个。其中,筛选出5个主要活性成分(槲皮素、山柰酚、柚皮素、异槲皮酚、异鼠李素)及6个核心靶点(蛋白激酶B1、肿瘤坏死因子、肿瘤蛋白P53、白细胞介素6、人雌激素受体1、白细胞介素1β),涉及肿瘤坏死因子信号通路、胰岛素抵抗、脂肪细胞因子信号通路等。分子对接分析发现,补中益气汤的核心活性成分与肌少症的关键靶点之间表现出较强的结合活性与稳定性。结论补中益气汤可通过多重活性成分、作用靶点及生物学通路对肌少症发挥潜在治疗作用。
Objective To explore the potential mechanism of Buzhong Yiqi decoction in the intervention of sarcopenia through network pharmacology methods.Methods The active components of seven herbs in Buzhong Yiqi decoction were screened using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).The targets of these active components were identified by leveraging TCMSP and the Swiss Target Prediction Database.Disease-related targets associated with sarcopenia were extracted from the disease database,and the intersection of targets related to the active components of the Chinese herbal compound and disease targets was obtained.Protein-protein interaction network analysis was performed,followed by Gene Ontology function analysis and Kyoto Encyclopedia of Genes and Genernes pathway enrichment analysis.Finally,molecular docking validation was conducted for the core components and key targets.Results A total of 182 active components and 1139 component targets of Buzhong Yiqi decoction were identified,including 415 disease-related targets associated with sarcopenia and 62 overlapping targets.Among these,5 major active components were screened,including quercetin,kaempferol,naringenin,isosquercetin,and isorhamnetin,as well as 6 core targets,including protein kinase B1,tumor necrosis factor,tumor protein P53,interleukin-6,human estrogen receptor 1,and interleukin-1β.These targets are involved in pathways such as the TNF signaling pathway,insulin resistance,and adipocytokine signaling pathways.Molecular docking analysis revealed that the core active components of Bu Zhong Yi Qi decoction exhibit strong binding activity and stability with key targets of sarcopenia.Conclusion Buzhong Yiqi decoction may exert potential therapeutic effects on sarcopenia through multiple active ingredients,target points and biological pathways.
作者
蒋佳慧
陈扬波
JIANG Jiahui;CHEN Yangbo(The Integrated Traditional Chinese and Western Medicine School of Clinical Medicine,Zhejiang Chinese Medical University,Hangzhou 310012,Zhejiang,China;Department of Critical Care MedicineⅡ,Tongde Hospital of Zhejiang Province,Hangzhou 310012,Zhejiang,China)
出处
《中国现代医生》
2026年第5期63-68,共6页
China Modern Doctor
关键词
补中益气汤
肌少症
网络药理学
分子对接技术
Buzhong Yiqi decoction
Sarcopenia
Network pharmacology
Molecular docking technology
