摘要
【目的】水通道蛋白(Aquaporin,AQP)是一种通道形成蛋白,促进水、甘油和其他小溶质穿过细胞膜的运输。掌握昆虫病原线虫AQP的生物学功能及其在昆虫病原线虫响应非生物协迫过程中的作用具有重要意义。【方法】本研究分析了昆虫病原线虫Steinernema carpocapsae的基因组和干燥及紫外线辐射胁迫下的转录组数据,从该线虫中预测到7个AQP基因,分别为L596_g7661、L596_g18121、XLOC_001193、XLOC_007750、DN12232、DN26938和DN25287,这些AQP均属于MIP超家族,具有6个跨膜螺旋结构域和2个高度保守的水通道蛋白特征序列Asn-Pro-Ala(NPA)。【结果】系统进化分析结果显示,L596_g7661、L596_g18121、XLOC_007750、DN12232和DN26938属于可渗透甘油的水甘油通道蛋白亚家族,XLOC_001193和DN25287属于水特异性的水通道蛋白亚家族。研究进一步对S.carpocapsae不同AQP的理化性质、保守基序及互作网络进行了分析。【结论】本研究分析了昆虫病原线虫S.carpocapsae的AQP基因家族的特征,为深入研究水通道蛋白在S.carpocapsae响应环境胁迫中的功能提供了依据,为昆虫病原线虫环境耐性分子机理的进一步研究提供了基础。
【Aim】Aquaporin(AQP)is a channel-forming protein that facilitates the transport of water,glycerol,and other small solutes across cell membranes.It is of great significance to grasp the ecological function of AQP in entomopathogenic nematodes and its role in the nematodes'reponse to abiotic stress.【Methods】In this study,the genome and transcriptome data in response to desiccation and ultraviolet radiation stress of the entomopathogenic nematode Steinernema carpocapsae were analyzed.Seven AQP genes were predicted from S.carpocapsae,which including L596_g7661,L596_g18121,XLOC_001193,XLOC_007750,DN12232,DN26938,and DN25287.【Results】Analysis showed that the predicted AQPs belonged to the MIP superfamily,which contained six transmembrane-spanningα-helices and a pair of highly conserved signature sequences,Asn-Pro-Ala(NPA)boxes.Phylogenetic analysis showed that L596_g7661,L596_g18121,DN12232,DN26938 and XLOC_007750 belonged to the glycerolpermeable aquaglyceroporin subgroup,while XLOC_001193 and DN25287 belonged to the waterpermeable aquaporin subgroup.The physical and chemical properties,conserved motifs,and interaction networks of different S.carpocapsae AQPs were also analyzed.【Conclusion】This study analyzed the characteristics of the AQP gene family in the entomopathogenic nematode S.carpocapsae,which provided a basis for the function study of the S.carpocapsae AQPs in response to environmental stress.The current study also provided the basis for the further study of the molecular mechanism of environmental tolerance in entomopathogenic nematodes.
作者
黄秋月
陈咏琦
丁倩
颜珣
HUANG Qiu-Yue;CHEN Yong-Qi;DING Qian;YAN Xun(College of Agriculture&Biology,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China,Ministry of Agriculture and Rural Affairs,Guangzhou 510225,China;Innovative Institute for Plant Health,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China)
出处
《环境昆虫学报》
北大核心
2026年第1期240-249,I0006,I0007,共12页
Journal of Environmental Entomology
基金
国家自然科学基金面上项目(32172491)
广东省重点建设学科科研能力提升项目(2022ZDJS020)
江门市2024年科技支撑“百千万工程”项目(KTP20240413)
云浮市科技计划项目(2022020415)。
