摘要
蔗糖合成酶(SUS)是植物蔗糖代谢的关键酶,参与调控产量、品质及逆境响应。本研究利用生物信息学方法对油茶SUS基因家族进行全基因组鉴定,并分析其染色体分布、进化关系、理化性质、基因结构、启动子顺式作用元件、共线性及低温胁迫下的表达模式。结果表明,共鉴定到20个CoSUS基因,不均匀分布于12条染色体上,可分为3个进化族。CoSUS蛋白均为亲水性,主要定位于细胞质和叶绿体;其启动子区域含有激素及胁迫响应相关顺式作用元件。共线性分析可知全基因组复制事件或串联重复可能是CoSUS基因进化的主要动力。qRT-PCR分析显示,10个CoSUS基因表达具有组织特异性,且在低温胁迫下多数基因表达呈先上调后下降的趋势,表明其参与低温胁迫响应。本研究为解析油茶SUS基因在低温胁迫中的分子机制及耐寒种质选育提供了重要依据。
Aims:Sucrose synthase(Sus)is central to plant sucrose metabolism,influencing growth,development,and stress responses.Camellia oleifera,a key woody oil crop,is susceptible to low-temperature stress.Although the SUs family has been studied in many plants,a systematic analysis in C.oleifera is absent,hindering understanding of its role in cold adaptation.This study aimed to perform a genome-wide identification and characterization of the CoSUS gene family,and to investigate their expression under cold stress,providing a foundation for molecular breeding of cold-tolerant varieties.Methods:We employed an integrated bioinformatics and molecular biology approach.All CoSUS genes were identified from the C.oleifera genome using HMMER and validated with NCBI CDD.Phylogenetic,gene structure,and motif analyses were conducted.Promoter cis-elements were predicted using Plant-CARE.Synteny analysis was performed with MCScanX to infer duplication events.The expression patterns of selected CoSUS genes in different tissues and under time-course low-temperature(4°C)treatment were analyzed by quantitative real-time PCR(qRT-PCR).Results:We identified 20 CoSUS genes unevenly distributed on 12 chromosomes.Phylogenetic analysis classified them into three conserved groups.These CoSUS proteins are all hydrophilic and primarily localized in the cytoplasm and chloroplasts.Gene structure and motif composition were group-specific.Promoter analysis revealed an abundance of hormone-and stress-responsive cis-elements.Synteny analysis indicated that whole-genome duplication was the primary driver for CoSUS family expansion.qRT-PCR results showed that ten CoSUS genes exhibited tissue-specific expression and most responded dynamically to cold stress,displaying a characteristic pattern of rapid up-regulation followed by decline.Conclusion:This study systematically characterizes the SUS gene family in C.oleifera for the first time.The expansion of the family is mainly driven by whole-genome duplication events.The cold-inducible expression patterns and stress-related cis-elements suggest that CoSus genes are important players in the cold stress response,likely through modulating sucrose metabolism.These findings offer valuable genetic resources and candidate genes for future functional studies aimed at enhancing cold tolerance in C.oleifera through molecular breeding.
作者
刘晚霞
郑佳
熊旖旎
张学一
李泽
谢雯慧
尹一安
吴玲利
LIU Wanxia;ZHENG Jia;XIONG Yini;ZHANG Xueyi;LI Ze;XIE Wenhui;YIN Yian;WU Lingli(State Key Laboratory of Woody Oil Resources Utilization,Central South University of Forestry and Technology,Changsha 410004,China;Yuelushan Laboratory,Central South University of Forestry and Technology,Changsha 410004,China;Hunan Chaling Camellia oleifera Science and Technology Backyard,Chaling,Hunan 412400,China)
出处
《植物生理学报》
北大核心
2026年第1期55-70,共16页
Plant Physiology Journal
基金
国家自然科学基金青年基金(32301640)
湖南省自然科学基金面上基金(2024JJ5638)
长沙市自然科学基金(kq2402257)
湖南省林业科技创新计划项目(湘财资环指[2024]3号)。
关键词
油茶
蔗糖合成酶
生物信息分析
低温胁迫
表达分析
Camellia oleifera
sucrose synthase
bioinformatics analysis
cold stress
expression analysis
