摘要
目的研究两色金鸡菊水提物对乙醇所致人肝癌HepG2细胞损伤以及KM小鼠急性肝损伤的保护作用,为两色金鸡菊水提物防治急性肝损伤提供依据。方法体外通过乙醇损伤HepG2肝细胞建立急性肝细胞损伤模型,以细胞存活率、肝功能和氧化应激指标评价两色金鸡菊水提物对HepG2细胞损伤的保护作用。体内将48只KM小鼠随机分为正常组(未处理)、模型组、阳性药组(水飞蓟宾)和两色金鸡菊水提物低(0.2 mg/kg)、中(0.4 mg/kg)、高(0.8 mg/kg)组,每组8只,连续给药7 d后,模型组和给药组通过腹腔注射0.5%CCl4橄榄油溶液建立急性肝损伤模型,测定小鼠肝功能指标、氧化应激及炎症因子指标。应用H&E染色法分析小鼠肝脏组织病理变化。结果两色金鸡菊水提物(50µg/mL)预孵育使乙醇诱导HepG2细胞存活率增加12.92%,丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)活力分别由模型组的20.93、27.33 U/L下降至15.91和19.32 U/L,超氧化物歧化酶(SOD)和还原性谷胱甘肽(GSH)活力分别提高10.02 U/mgprot和34.57µmol/gprot,丙二醛(MDA)含量下降3.64 nmol/mgprot。与正常组比较,模型组小鼠血清ALT、AST活性(t=33.52、29.95),肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)水平,肝组织MDA水平(t=46.72、22.86、21.48)均明显上升,肝组织SOD、GSH活力(t=26.24、6.98)均明显下降,差异均有统计学意义(P均<0.05);与模型组比较,两色金鸡菊水提物低、中、高剂量组小鼠血清ALT(t=4.45、6.85、9.64)、AST(t=4.13、5.48、7.38)、TNF-α(t=22.78、24.20、31.11)、IL-6(t=6.63、7.41、9.89)水平,肝组织MDA(t=6.01、9.67、13.81)水平均明显下降,肝组织SOD(t=9.47、10.98、13.32)、GSH(t=3.05、5.46、7.45)活力均明显上升,差异均有统计学意义(P均<0.05)。结论两色金鸡菊水提物在急性肝损伤的保护方面具有一定的作用,可能与减轻氧化应激状态和炎症反应有关。
Objective To investigate the protective effects of the aqueous extract of Coreopsis tinctoria(AECT)against ethanol-induced injury in human hepatoma HepG2 cells and acute liver injury in KM mice,providing a basis for the potential use of AECT in preventing and treating acute liver injury.Methods In vitro,an acute liver injury model was established by inducing ethanol damage in HepG2 hepatocellular carcinoma cells.The protective effect of Coreopsis tinctoria aqueous extract on HepG2 cell injury was evaluated based on cell viability,liver function,and oxidative stress indicators.In vivo,48KM mice were randomly divided into six groups(n=8 per group):normal control(untreated),model group,positive control group(silymarin-treated),and low-(0.2 mg/kg),medium-(0.4 mg/kg),and high-dose(0.8 mg/kg)Coreopsis tinctoria aqueous extract groups.After 7 consecutive days of administration,the model and treatment groups received an intraperitoneal injection of O.5%CCl4 olive oil solution to establish an acute liver injury model.Liver function markers,oxidative stress parameters,and inflammatory cytokines were measured.Hepatic histopathological changes were analyzed using H&E staining.Results Pre-incubation with AECT(50µg/mL)increased the viability of ethanol-injured HepG2cells by 12.92%.Alanine aminotransferase(ALT)and aspartate aminotransferase(AST)activities decreased from 20.93and 27.33 U/L in the model group to 15.91 and 19.32 U/L,respectively.Superoxide dismutase(SOD)and reduced glutathione(GSH)activities increased by 10.02 U/mgprot and 34.57μmol/gprot,respectively,while malondialdehyde(MDA)content decreased by 3.64 nmol/mgprot.Compared with the normal group,the model group showed significantly increased serum ALT and AST activities(t=33.52,29.95),tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6)levels,and liver tissue MDA content(t=46.72,22.86,21.48),along with significantly decreased liver tissue SOD and GSH activities(t=26.24,6.98)(all P<0.05).Compared to the model group,the low-,medium-,and high-dose AECT groups exhibited significant decreases in serum ALT(t=4.45,6.85,9.64),AST(t=4.13,5.48,7.38),TNF-α(t=22.78,24.20,31.11),IL-6(t=6.63,7.41,9.89)levels,and liver tissue MDA content(t=6.01,9.67,13.81),along with significant increases in liver tissue SOD(t=9.47,10.98,13.32)andGSH(t=3.05,5.46,7.45)activities(all P<0.05).Conclusion The aqueous extract of Coreopsis tinctoria exhibited protective effects against acute liver injury,which might be associated with alleviating oxidative stress and suppressing inflammatory responses.
作者
王红伊
日萨莱提·亚森
陶义存
WANG Hongyi;RISALAT Yasin;TAO Yicun(School of Pharmacy,Xinjiang Medical University,Urumqi,Xinjiang 830017,China)
出处
《热带医学杂志》
2026年第1期11-16,22,F0003,共8页
Journal of Tropical Medicine
基金
新疆维吾尔自治区重点研发项目(2023B02010-4)。
关键词
两色金鸡菊
急性肝损伤
抗氧化
Coreopsis tinctoria
Acute liver injury
Antioxidant
