摘要
金属硫蛋白(metallothionein,MT)是一类低分子量蛋白。目前,已有大量关于植物吸收和调控重金属Cd积累的分子机制的研究,然而研究结果相对来说不够系统,主要集中于部分转运蛋白的研究。为系统揭示金属硫蛋白在水稻调控网络中的潜在功能,并弥补现有研究在基因家族层面系统性的不足的缺口,本研究通过生物信息学分析,共鉴定到14个水稻金属硫蛋白基因家族成员,水稻金属硫蛋白基因家族成员的motif有较大差异,蛋白序列的进化树分析将其分为4类亚家族,其中OsMT1.4A、OsMT1.4B、OsMT1.4C、OsMT1.1C、OsMT1.1G串联成簇分布在12号染色体上。预测的顺式元件与植物激素、生长、环境、光响应相关。大部分的金属硫蛋白受各类激素诱导表达,镉胁迫诱导其中10个成员的表达量上调。OsMT1.3A、OsMT1.3B保守结构域与其他OsMTs差异较大,表达模式与其他家族基因截然不同。异源表达OsMT1.3A、OsMT1.4A显著提高了细菌和酵母对Cd的耐受性,水稻中过表达OsMT1.4A显著增加了有效穗数和产量。本研究为通过生物技术的手段培育Cd耐受并具有修复Cd污染潜力的农作物提供了优异的基因资源。
Metallothionein(MT)is a low molecular weight protein.At present,a large number of studies on the molecular mechanisms of plant uptake and regulation of heavy metal Cd accumulation have been carried out.However,the research results obtained are relatively not systematic enough,mainly focusing on the study of some transporter proteins.To bridge the existing gap in systematic understanding of the metallothionein gene family,we aimed to uncover its potential functions within the rice regulatory network.Through bioinformatics analysis,a total of 14 rice MT gene family members were identified.The motif structure prediction indicated that there were great differences among members of this family,which could be classified into 4 subfamilies by phylogenetic analysis of protein sequences.OsMT1.4A,OsMT1.4B,OsMT1.4C,OsMT1.1C,and OsMT1.1G existed in series clusters on the 12th chromosome.The predicted ciselements were related to plant hormones,growth,environment,and light responses.The expression of most members was induced by various plant hormones,and the expression of 10 members was upregulated by cadmium(Cd)stress.The OsMT1.3A and OsMT1.3B subfamilies showed greatly differed conserved domains from other OsMTs genes,and their expression patterns were completely different from those of other families.Heterologous expression of OsMT1.3A and OsMT1.4A significantly improved the tolerance of bacteria and yeast to Cd,and overexpression of OsMT1.4A in rice significantly increased the effective panicle number and yield.This study provides excellent genetic resources for breeding Cd-tolerant crops with potential for remediation of Cd pollution by biotechnology.
作者
苗渝青
张超
韩子静
翟雨航
张文祺
金锐
张振华
陈海飞
MIAO Yuqing;ZHANG Chao;HAN Zijing;ZHAI Yuhang;ZHANG Wenqi;JIN Rui;ZHANG Zhenhua;CHEN Haifei(College of Resources,Hunan Agricultural University,Changsha 410128,Hunan,China;Yuelushan Laboratory,Changsha 410128,Hunan,China)
出处
《生物工程学报》
北大核心
2026年第1期288-302,共15页
Chinese Journal of Biotechnology
基金
国家自然科学基金(32172669)
湖南省科技创新计划(2024RC3179)。
关键词
水稻
微生物
镉胁迫
金属硫蛋白基因家族
表达模式
rice
microorganism
cadmium stress
metallothionein(MT)gene family
expression pattern
