摘要
微生物氨氧化过程在水体质量、渔业资源可持续利用以及氮素生物地球化学循环中起到关键作用。以升钟湖沉积物为研究对象,采用室内微宇宙培养实验,结合13 C-CO 2稳定性同位素核酸探针(DNA-Stable Isotope Probing,DNA-SIP)技术和高通量测序,系统揭示了沉积物中参与氨氧化过程的活性氨氧化微生物群落。通过对超高速密度梯度离心获得的重层被标记的13 C-DNA,对氨氧化细菌(AOB)、氨氧化古菌(AOA)和全过程氨氧化菌(Comammox)的amoA基因,以及厌氧氨氧化菌(Anammox)的hzs-β基因进行荧光定量PCR发现,AOB、Comammox和Anammox的DNA被13 C显著标记。对13 C-DNA测序发现,参与该沉积物中氨氧化的AOB有12个ASV,主要为隶属于亚硝化单胞菌科(Nitrosomonadaceae)的亚硝化螺菌属(Nitrosospira);Comammox有4个ASV,主要为硝化螺菌属(Nitrospira)的Nitrospira calida分支;Anammox仅1个ASV,隶属于Candidatus Brocadia,与Candidatus Anammoximicrobium moscowii聚类在同一分支。这些结果表明,在缺氧环境下升钟湖沉积物中的氨氧化过程由多种微生物群落共同驱动。
Microbial ammonia oxidation plays a key role in water quality,sustainable utilization of fishery resources,and the biogeochemical cycle of nitrogen.This study conducted a microcosm experiment with sediments collected from Shengzhong Lake,combined with 13 C-CO 2 DNA-Stable Isotope Probing technique and high-throughput sequencing,to systematically identify the active ammonia-oxidizing microbial communities involved in ammonia oxidation process in the sediments.Through fluorescence PCR analysis of 13 C-DNA obtained via ultracentrifugation of density gradients,the amoA genes of ammonia-oxidizing bacteria(AOB),archaea(AOA),and Comammox,as well as the hzs-βgenes of anammox bacteria,respectively,it is observed that the DNA of AOB,Comammox,and anammox bacteria is significantly 13 C-labeled.The sequencing of the 13 C-DNA reveals that there are 12 ASVs of AOB involved in ammonia oxidation in this sediment,primarily belonging to Nitrosospira within the Nitrosomonadaceae family.For Comammox,there are 4 ASVs,mainly associated with the Nitrospira calida lineage of the genus Nitrospira.Anammox has only 1 ASV,which belongs to the genus Candidatus Brocadia and is clustered together with Candidatus Anammoximicrobium moscowii.These findings indicate that ammonia oxidation process in the sediments of Shengzhong Lake under anoxic conditions is driven by diverse ammonia-oxidizing microbial groups.
作者
张露文
黄丽萍
彭超
ZHANG Luwen;HUANG Liping;PENG Chao(College of Life Science,China West Normal University,Nanchong Sichuan 637009,China)
出处
《西华师范大学学报(自然科学版)》
2026年第2期136-143,共8页
Journal of China West Normal University(Natural Sciences)
基金
国家自然科学基金项目(42107278)
四川省科技厅应用基础研究项目(2022YEN0065)
西华师范大学科研创新团队项目(CXTD2020-3)。
关键词
氨氧化作用
湖泊生态系统
氨氧化微生物
同位素示踪技术
氮循环
ammonia oxidation
lake ecosystem
ammonia-oxidizing microorganism
stable-isotope-tracer technique
nitrogen cycling
