摘要
建立了三氯乙酸-双缩脲分光光度法测定肥料中鱼蛋白肽含量的方法。肥料样品用体积分数15%的三氯乙酸溶液预处理30 min后,采用紫外-可见分光光度计于540 nm处测定溶液的吸光度。结果表明:在0~1.0 mg/mL范围内,鱼蛋白肽质量浓度与吸光度呈良好的线性关系,R^(2)=0.9994;方法的加标回收率为95.0%~104.8%,10次平行测定结果的相对标准偏差为1.99%。三氯乙酸-双缩脲分光光度法具有良好的精密度与准确度,适用于肥料中鱼蛋白肽含量的测定。
A trichloroacetic acid-biuret spectrophotometry is established for the determination of fish protein peptide content in fertilizers.After pre-treating the fertilizer sample with 15%(volume fraction)trichloroacetic acid solution for 30 min,the absorbance of the solution is measured at 540 nm using ultraviolet-visible spectrophotometre.The results show that the fish protein peptide mass concentration exhibited a good linear relationship with absorbance within the range of 0-1.0 mg/mL,with R^(2)=0.9994.The spiked recovery rate of the method is 95.0%to 104.8%,and the relative standard deviation of the result from 10 parallel determinations is 1.99%.The trichloroacetic acid-biuret spectrophotometry offers good precision and accuracy,making it suitable for the determination of fish protein peptide content in fertilizers.
作者
宋海妹
刁红霞
张超一
台文静
张静
王程程
雷芳
SONG Haimei;DIAO Hongxia;ZHANG Chaoyi;TAI Wenjing;ZHANG Jing;WANG Chengcheng;LEI Fang(Qingdao Seawin Biotech Group Co.,Ltd.,Qingdao,Shandong 266000,China)
出处
《肥料与健康》
2025年第6期59-61,67,共4页
Fertilizer & Health
基金
山东省重点研发计划(科技示范工程)项目(2024SFGC0403)。
关键词
肥料
鱼蛋白肽
三氯乙酸-双缩脲
分光光度法
fertilizer
fish protein peptide
trichloroacetic acid-biuret
spectrophotometry
