摘要
保幼激素(juvenile hormone,JH)是执行内分泌调节的重要激素之一,在节肢动物的生殖发育和变态过程中通过与其相关受体结合发挥重要作用。为了探究保幼激素相关受体基因Met在孤雌卤虫(Artemia)卵巢发育过程中的作用,本研究对ApMet基因(孤雌卤虫保幼激素相关受体基因Met)进行相关生物信息学分析,采用实时荧光定量逆转录聚合酶链反应(q RT-PCR)验证该基因在卤虫卵巢、脑神经节和肠道组织中的时空表达特征。结果显示,ApMet基因的开放阅读框全长1 476 bp,编码419个氨基酸;该基因具有节肢动物中4个保守的典型结构域,分别是bHLH、PAS-A、PAS-B和PAC,ApMet蛋白的三级结构主要包含无规则卷曲和α-螺旋,二级结构相似;在系统发育树中,孤雌卤虫与溞状蚤(Daphnia pulex)、大型蚤(Daphnia magna)最为相近,与德国小蠊(Blattella germanica)等昆虫的亲缘关系较远。基因表达特征分析结果显示,ApMet在肠道中表达量最高,在卵巢中次之,且在卵巢发育过程中呈差异性表达,表明该基因可能是多功能基因且参与调节卤虫的卵巢发育过程。本研究为甲壳动物Met基因的结构及生理功能提供参考和依据。
Juvenile hormone(JH)is one of the key hormones involving in endocrine regulation,playing crucial roles in reproductive development and metamorphosis of arthropods through its associated receptors.To investigate the role of juvenile hormone-related receptor Met(Methoprene-tolerant)gene in the ovarian development of parthenogenetica Artemia,this study conducted bioinformatics analysis of the ApMet gene(juvenile hormone related receptor gene Met in parthenogenetica Artemia)and applied quantitative reverse transcription PCR(qRT-PCR)to examine its spatial and temporal expres sion patterns in ovary,cerebral ganglion and intestine tissues.The results revealed that the ORF of ApMet gene contained 1476 bp,encoding a protein of 419 amino acids.Four conserved domains,namely bHLH,PAS-A,PAS-B,and PAC,were possessed by the ApMet gene.The tertiary structure prediction of ApMet protein included irregular coils andα-helices,consistent with the secondary structure prediction results.In the phylogenetic tree,Daphnia pulex and Daphnia magna were identified as the closest relatives to Parthenogenetic Artemia,while a more distant relationship was observed with insects Blattella germanica.Gene expression analysis showed that ApMet was most highly expressed in the intestine,followed by ovary,and exhibited differential expression during ovarian development.This study provides valuable insights into the gene structure and physiological functions of Met gene in crustaceans.
作者
刘伟
穆民
李志腾
隋丽英
LIU Wei;MU Min;LI Zhiteng;SUI Liying(College of Marine and Environmental Sciences,Tianjin University of Science and Technology,Tianjin 300457,China;Beijing Gas Group(Tianjin)Liquefied Natural Gas Co.,Ltd.,Tianjin 300280,China)
出处
《天津科技大学学报》
2026年第1期37-43,共7页
Journal of Tianjin University of Science & Technology
基金
海洋资源与食品化学教育部重点实验室(EMTUST-25-06)
天津市种业重大创新专项(24ZXZYSN0020)。
