摘要
双酚A(bisphenol A,BPA)是一种广泛应用于工业的有机化合物,常用于合成聚碳酸酯和环氧树脂等材料。BPA在生产和生活中的广泛应用导致其在水环境中被频繁检出,对水生生物的健康构成潜在威胁。相关研究证实BPA对水生生物的生殖和发育具有一定的毒性效应,但对鱼类肠道的毒性效应研究却十分有限。本研究将稀有鮈鲫(Gobiocypris rarus)暴露于15μg·L^(-1)BPA中7、14和21 d,通过组织切片、实时荧光定量PCR和16S rRNA测序等技术探究了稀有鮈鲫肠道菌群和形态结构变化。结果表明,BPA暴露引起稀有鮈鲫肠道组织出现杯状细胞增生和肠绒毛缩短等现象,且随着暴露时间的延长肠道组织损伤情况更加明显。BPA暴露引起稀有鮈鲫肠道菌群组成发生变化,门水平上,BPA暴露引起衣原体门和芽孢杆菌门的相对丰度上升。属水平上,生丝微菌属、新衣原体属和芽殖杆菌属的占比在BPA暴露后上升,这表明BPA暴露可能会破坏稀有鮈鲫肠道微生物群稳态。此外,BPA暴露干扰了IL-6、IL-1β、TNF-α和IL-10等炎症相关基因的表达,表明BPA暴露可能在一定程度上导致稀有鮈鲫肠道出现炎症反应。紧密连接蛋白基因的表达在BPA暴露后呈现不同程度的响应,BPA暴露7 d和21 d后引起稀有鮈鲫肠道Claudin 3基因的相对表达量显著下调(P<0.05);Occludin基因在BPA暴露的14 d和21 d后显著下调(P<0.05);ZO-1基因在暴露周期内无显著变化。以上结果表明,BPA暴露会导致肠道菌群紊乱和炎症反应,进而影响鱼类肠道健康。
Bisphenol A(BPA),a synthetic compound used in the manufacture of polycarbonates and epoxy resins,is frequently detected in aquatic environments due to its extensive industrial and domestic use.Although BPA’s reproductive and developmental toxicity in aquatic species is well documented,its effects on fish intestinal health remain poorly understood.Here,we exposed Gobiocypris rarus to 15μg·L^(-1)BPA for 7,14,and 21 days,and assessed intestinal histopathology,gene expression,and microbiota composition using histology,quantitative PCR,and 16S rRNA sequencing.BPA exposure induced marked pathological changes in the intestinal tissue of Gobiocypris rarus,including goblet cell hyperplasia and villus shortening,with damage severity increasing over time.The gut microbiota composition was significantly altered,with elevated relative abundances of Chlamydiota and Bacillota at the phylum level,and Hyphomicrobium,Neochlamydia,and Gemmobacter at the genus level,indicating disruption of microbial homeostasis.BPA also modulated the expression of inflammation-related genes,up-regulating IL-6,IL-1β,TNF-α,and IL-10,suggesting the induction of intestinal inflammatory responses.Tight junction protein expression responded differentially:Claudin 3 was significantly down-regulated at 7 and 21 days(P<0.05),while Occludin was significantly down-regulated at 14 and 21 days(P<0.05).The ZO-1 gene showed no significant changes throughout the exposure period.Collectively,these results demonstrate that BPA exposure induces intestinal dysbiosis,promotes inflammation,and impairs barrier integrity,ultimately compromising gut health in fish.
作者
王骏
刘正元
李欣蕊
董健皓
许星鸿
朱龙
WANG Jun;LIU Zhengyuan;LI Xinrui;DONG Jianhao;XU Xinghong;ZHU Long(School of Marine Science and Fisheries,Jiangsu Ocean University,Lianyungang 222000,China;Jiangsu Institute of Marine Resources Development,Jiangsu Ocean University,Lianyungang 222000,China)
出处
《生态毒理学报》
北大核心
2026年第1期389-398,共10页
Asian Journal of Ecotoxicology
基金
国家自然科学基金青年项目(32401405)
江苏省自然科学基金青年项目(BK20230690)
江苏省高等学校自然科学研究项目(23KJB180005)
江苏海洋大学研究生科研与实践创新计划项目(KYCX2025_27)。
关键词
双酚A
稀有鮈鲫
肠道菌群
炎症反应
bisphenol A
Gobiocypris rarus
intestinal microbiota
inflammation
