摘要
[目的]探究P2RX7调控PI3K/AKT通路对丙泊酚麻醉后导致海马神经元损伤的影响。[方法]将C57BL/6小鼠随机平分为4组:空白组、损伤组、P2RX7 siRNA组和740Y-P组。空白组、损伤组尾静脉注射100μL/只的空载腺病毒,P2RX7 siRNA组注射100μL/只P2RX7 siRNA腺病毒,740Y-P组每只10 mg/kg注射740Y-P。采用苏木素染色法评估小鼠海马组织的病理变化;原位末端标记法检测小鼠海马神经元的凋亡率;水迷宫实验分析小鼠穿越平台次数以及逃避潜伏期时长;酶联免疫吸附实验分析小鼠体内炎性细胞因子水平;蛋白免疫印迹实验检测小鼠海马组织P2RX7、PI3K、AKT蛋白的表达。[结果]与损伤组小鼠比较,P2RX7 siRNA组以及740Y-P组小鼠的海马组织损伤程度减轻、海马神经元凋亡率降低(P<0.05)、小鼠穿越平台次数增加(P<0.05)、逃避潜伏期缩短(P<0.05)、炎性细胞因子释放减少,PI3K、AKT蛋白表达增加(P<0.05)。[结论]下调P2RX7可激活PI3K/AKT通路进而改善丙泊酚麻醉后导致的海马组织损伤和认知功能障碍,降低神经元凋亡和炎症因子的释放水平。
[Objective]To investigate the effect of P2RX7 on hippocampal neuron injury induced by propofol anesthesia by regulating PI3K/AKT pathway.[Method]The C57BL/6 mice were randomly divided into(10 mice in each group):the con-trol group,the injury group,the P2RX7 siRNA group,and the 740Y-P group.The empty adenovirus was injected into the mice in the control and injury groups via the tail vein(100μL per mouse),the P2RX7 siRNA adenovirus was injected into the mice in the P2RX7 siRNA group(100μL per mouse),and 740Y-P was injected into the mice in the 740Y-P group(10 mg/kg per mouse).Hematoxylin-eosin staining was used to analyze the pathological changes in the hippocampus.The apopto-sis rate of hippocampal neurons was detected by in situ end labeling.Water maze test was used to evaluate the changes in the number of crossing the platform and the escape latency time.Enzyme-linked immunosorbent assay was used to analyze the lev-els of inflammatory factors in the hippocampus of mice.The protein expression levels of P2RX7,PI3K and AKT in the hippo-campus of mice were analyzed by Western blot.[Result]Compared with the injury group,the P2RX7 siRNA group and the 740Y-P group had significantly reduced hippocampal tissue damage and hippocampal neuron apoptosis rate(P<0.05),sig-nificantly increased the number of platform crossing(P<0.05),significantly shortened the escape latency(P<0.05),signif-icantly reduced the release of inflammatory factors in the hippocampus,and significantly increased the expression of PI3K and AKT proteins(P<0.05).[Conclusion]Down-regulation of P2RX7 can improve hippocampal tissue damage and cognitive dysfunction caused by propofol anesthesia by activating the PI3K/AKT pathway,and reduce neuronal apoptosis and the release levels of inflammatory factors.
作者
石可
毛凯
SHI Ke;MAO Kai(Department of Anesthesiology,The Second Affiliated Hospital of Hubei University of Science and Technology,Xianning 437000,China)
出处
《生物技术》
2025年第6期749-753,697,共6页
Biotechnology
