摘要
为解决刺参(Apstichopusjaponicus)养殖中细菌性疾病日趋凸显及抗生素不当使用带来的安全隐患与耐药性问题,研究评估了抗菌肽(Antimicrobial peptide,AMPs)A7在刺参养殖中对弧菌(Vibrio)的抑制效果及应用潜力。本研究以30株水产养殖来源的弧菌为指示菌,采用牛津杯法和96孔板法评价了其体外抑菌活性,并将其按0、3、5、10 mg·L^(-1)的用量均匀泼洒于刺参养殖池中,测定了第0、第24、第48小时水体弧菌的数量变化。结果显示,抗菌肽A7对30株弧菌均有抑制效果,对灿烂弧菌(V.splendidus)的抑制效果最好,平均抑菌圈直径为25.3 mm,最小抑菌浓度(Minimum inhibitory concentration,MIC)为97 mg·L^(-1);而池塘有效剂量远低于实验室测定的MIC,3~10 mg·L^(-1)的抗菌肽A7对刺参养殖池弧菌均有较好的去除作用,使用剂量越高起效越快、去除率越高。综上所述,抗菌肽A7对水产养殖弧菌具有良好的抑制作用和防治应用前景。
To address the growing prevalence of bacterial diseases in sea cucumber farming and the safety concerns and antibiotic resistance issues arising from inappropriate antibiotic use,we evaluated the inhibitory effect and application potential of antimicrobial peptide(AMPs)A7 against Vibrio and its effectiveness in removing Vibrio from the aquaculture water of sea cucumber(Apstichopus japonicus).The antibacterial profile of A7 was determined by the Oxford cup antibacterial coil method and 96-well microtiter plates with 30 strains of Vibrio as the indicator bacteria.Thereafter,a pond trial was conducted to investigate different water concentrations of A7(0,3,5 and 10 mg·L^(-1)water in pond)in A.japonicus breeding pool.And Vibrio counts changes in water at 0th,24th and 48th hour were measured.The results show that A7 had inhibitory effects on 30 strains of Vibrio in the experiment,and the inhibitory effect on V.splendidus was best,with an average inhibition zone diameter of 25.3 mm and a minimum inhibitory concentration(MIC)of 97 mg·L^(-1).However,the effective dose in the pond was much lower than the MIC determined in the laboratory.Using 3–10 mg·L^(-1)antimicrobial peptide A7 had a good removal effect on Vibrio in sea cucumber ponds,and the higher the dose used,the faster the onset of effect and the higher the removal rate of Vibrio.In conclusion,antimicrobial peptide A7 shows broad spectrum bacteriostasis against Vibrio from aquatic aquaculture environment and holds promising prospects for prevention and control applications.
作者
梁晶晶
刘宝同
孟凡奎
刘洁
肖志壮
田相利
郭衍林
Liang Jingjing;Liu Baotong;Meng Fankui;Liu Jie;Xiao Zhizhuang;Tian Xiangli;Guo Yanlin(Qingdao GBW Groups Co.,Ltd,Qingdao 266111,China;Key Laboratory of Mariculture,Ministry of Education(Ocean University of China),Qingdao 266003,China)
出处
《南方水产科学》
北大核心
2026年第1期169-177,共9页
South China Fisheries Science
基金
青岛市海洋科技创新专项(24-1-3-hygg-26-hy)。
关键词
抗菌肽
刺参养殖
弧菌
体外抑菌活性
最小抑菌浓度
Antimicrobial peptide
Apstichopus japonicus aquaculture
Vibrio
In vitro antibacterial activity
Minimum inhibitory concentration
