摘要
为探究光氮互作对格木(Erythrophleum fordii)幼苗氮素利用和碳氮代谢的调控机制,以1年生格木幼苗为试验材料,设置4个氮素水平(0、2000、4000和6000毫克/株)和4种光照强度[100%自然光(Natural Sunlight,NS)、51.6%NS、25.5%NS和9.3%NS],采用双因素试验,探究光氮互作对格木幼苗生物量、氮含量、氮代谢关键酶活性、非结构性碳水化合物含量和可溶性蛋白含量的影响。结果表明,光照强度对格木幼苗茎和叶生物量,根、茎和叶氮和非结构性碳水化合物含量,氮代谢关键酶活性及可溶性蛋白含量均影响最大。在同一氮素条件下,随光照强度减弱,L0N0~L3N0、L0N1~L3N1、L0N2~L3N2和L0N3~L3N3处理根、茎和叶生物量及硝酸还原酶和谷氨酰胺合成酶活性总体均呈先升后降的趋势。L0N0~L3N0、L0N1~L2N1、L0N2~L3N2和L1N3~L3N3处理氮含量均表现为叶>茎>根。在同一氮素条件下,随光照强度减弱,L0N0~L3N0、L0N1~L3N1、L0N2~L3N2和L0N3~L3N3处理叶非结构性碳水化合物含量总体均呈下降趋势,L0N0~L3N0、L0N1~L3N1、L0N2~L3N2和L0N3~L3N3处理根和茎非结构性碳水化合物含量变化均无一致规律;L0N0~L3N0处理可溶性蛋白含量升高,L0N1~L3N1、L0N2~L3N2和L0N3~L3N3处理可溶性蛋白含量均呈先升后降的趋势。隶属函数分析结果表明,L1N2(51.6%NS和4000毫克/株氮肥)处理格木幼苗表现最优。
In order to investigate regulatory mechanism of light and nitrogen interaction on nitrogen utilization and carbon-nitrogen metabolism in Erythrophleum fordii seedlings,taking one-year-old E.fordii seedlings as experimental materials,two-factor experiment was conducted with four nitrogen levels(0,2000,4000 and 6000 mg/plant)and four light intensities[100%natural sunlight(NS),51.6%NS,25.5%NS and 9.3%NS].Effects of light and nitrogen interaction on biomass,nitrogen content,activities of key nitrogen metabolism enzymes,contents of non-structural carbohydrate and soluble protein of E.fordii seedlings were examined.Results showed that light intensity had the greatest influences on biomass of stems and leaves,as well as on contents of nitrogen and non-structural carbohydrate of roots,stems and leaves,activities of key nitrogen metabolism enzymes and soluble protein content of E.fordii seedlings.Under the same nitrogen condition,with decrease of light intensity,biomass of roots,stems and leaves,as well as activities of nitrate reductase and glutamine synthetase generally showed trends of firstly increase and then decrease in L0N0-L3N0,L0N1-L3N1,L0N2-L3N2 and L0N3-L3N3 treatments.In L0N0-L3N0,L0N1-L2N1,L0N2-L3N2 and L1N3-L3N3 treatments,orders of nitrogen contents were leaves>stems>roots.Under the same nitrogen condition,with decrease of light intensity,non-structural carbohydrate contents of leaves generally showed trends of decrease in L0N0-L3N0,L0N1-L3N1,L0N2-L3N2 and L0N3-L3N3 treatments,while changes in non-structural carbohydrate contents of roots and stems showed no the same patterns.Under the same nitrogen condition,with decrease of light intensity,soluble protein content increased in L0N0-L3N0 treatments,whereas changes of soluble protein contents showed trends of firstly increase and then decrease in L0N1-L3N1,L0N2-L3N2 and L0N3-L3N3 treatments.Result of membership function analysis indicated that E.fordii seedlings performed best in L1N2 treatment(51.67%NS and 4000 mg/plant nitrogen fertilizer).
作者
朱歆雨
黄玲璞
赵俊辉
田昱
桂嘉豪
王凌晖
黄雪蔓
Zhu Xinyu;Huang Lingpu;Zhao Junhui;Tian Yu;Gui Jiahao;Wang Linghui;Huang Xueman(College of Forestry,Guangxi University,Guangxi Key Laboratory of Forest Ecology and Conservation,Key Laboratory of National Forestry and Grassland Administration on Fast-growing Timber in Central South China,Nanning,Guangxi 530004,China;Nanning Greening Project Management Centre,Nanning,Guangxi 530012,China;Guangxi State-owned Qipo Forest Farm,Nanning,Guangxi 530225,China)
出处
《广西林业科学》
2025年第6期673-683,共11页
Guangxi Forestry Science
基金
中央财政林业草原科技推广示范项目([2023]TG 23号)
广西自然科学基金面上项目(2025GXNSFAA069288)。
关键词
光氮互作
生物量
氮素
生理生化特性
格木
light and nitrogen interaction
biomass
nitrogen
physiological and biochemical characteristics
Erythrophleum fordii
