摘要
溶解性有机质(DOM)是水生生态系统和全球碳循环的关键组分,但淡水内源DOM参考样品的缺乏,制约了对其环境行为和生物地球化学循环的深入理解.本研究旨在提供一种克级高纯度淡水内源DOM参考样品.通过放大的改性苯乙烯-二乙烯基苯聚合物树脂填充柱固相萃取法,处理南水北调中线工程8t河水,成功制备了13.12g DOM,命名为NSBD-DOM.综合运用元素分析、傅立叶变换红外光谱、三维荧光光谱、核磁共振波谱和高分辨质谱等手段,系统表征了NSBD-DOM的化学组成,并与国际腐殖酸协会提供的苏万尼河富里酸(SRFA)进行对比.结果表明,NSBD-DOM主体为类富里酸组分,包含碳水化合物端基碳、烯烃/共轭烯烃以及氨基糖等特征.相较于SRFA,NSBD-DOM的分子量、缩合度、氧元素含量较低,其中多酚类和多环芳烃类化合物含量较低;然而,其氢碳比、氮含量、不饱和脂肪族化合物和高度不饱和化合物含量较高.NSBD-DOM具备典型内源特征,可为DOM的环境行为、碳循环等研究提供参考.
Dissolved organic matter(DOM)is a key component of aquatic ecosystems and the global carbon cycle.However,the lack of reference samples for endogenous freshwater DOM has hindered a deeper understanding of its environmental behavior and biogeochemical cycling.This study aims to provide a gram-scale,high-purity endogenous freshwater DOM reference sample.By employing an amplified solid-phase extraction method using modified styrene-divinylbenzene polymer resin-packed columns,8tons of water from the central line project of South-to-North Water Diversion were processed,successfully yielding 13.12g of DOM,named as NSBD-DOM.A comprehensive suite of analytical techniques—including elemental analysis,Fourier transform infrared spectroscopy,excitation-emission matrix spectra,nuclear magnetic resonance spectroscopy,and high-resolution mass spectrometry—was employed to systematically compare NSBD-DOM with Suwannee River Fulvic Acid(SRFA)from the International Humic Substances Society.The NSBD-DOM was predominantly composed of fulvic acid like components,exhibiting characteristic of carbohydrate anomeric carbon,alkenes/conjugated alkenes,and amino sugars.Compared to SRFA,NSBD-DOM exhibited lower molecular weight,condensation degree,and oxygen content,accompanied by lower levels of polyphenols and polycyclic aromatics.However,it showed a higher H/C ratio and higher nitrogen content,with elevated levels of unsaturated aliphatic compounds and highly unsaturated compounds.NSBD-DOM exhibits typical endogenous characteristics,it can serve as a unified reference for studies on DOM’s environmental behavior,carbon cycling,and related research fields.
作者
胡浩杰
何晨
潘娜
王飏荣
王一达
宋桂雪
白雪松
阮振鑫
温佳鑫
王亚琦
史权
HU Hao-jie;HE Chen;PAN Na;WANG Yang-rong;WANG Yi-da;SONG Gui-xue;BAI Xue-song;RUAN Zhen-xin;WEN Jia-xin;WANG Ya-qi;SHI Quan(State Key Laboratory of Heavy Oil Processing,China University of Petroleum,Beijing 102249,China;Beijing Branch,China South to North Water Diversion Group Mid Route Co.Ltd.,Beijing 102408,China;Institute of Marine Science and Technology,Shandong University,Qingdao 266237,China)
出处
《中国环境科学》
北大核心
2026年第2期888-897,共10页
China Environmental Science
基金
国家重点研发计划项目(2020YFA0607600)
中国石油大学(北京)科学基金资助项目(2462023YJRC003)。
关键词
溶解性有机质
大体积固相萃取
PPL树脂
南水北调
dissolved organic matter
large-scale extraction
PPL resin
south-to-north water diversion
