摘要
目的探讨参芪补气颗粒(SQBQ)对慢性心衰大鼠的治疗作用及配伍科学性。方法SPF级雄性SD大鼠随机分为假手术组、模型组、参芪补气颗粒组(6.93 g/kg)、人参组(0.63 g/kg)、黄芪组(6.30 g/kg)和福辛普利钠组(1.05 mg/kg)。采用大鼠心梗后心衰模型,连续灌胃给药28 d,超声检测大鼠心功能与结构,观察心肌病理变化,检测心肌酶、心衰标志物及炎症因子等血液学指标,并进行心肌组织代谢组学分析。结果与假手术组相比,模型组大鼠心室结构异常,舒缩功能障碍,心功能显著下降(P<0.01);心肌细胞水肿坏死,炎细胞大片浸润,纤维化明显;血清肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、心肌肌钙蛋白T(cTnT)及N末端脑钠肽前体(NT-proBNP)均显著升高(P<0.05);血浆超氧化物歧化酶(SOD)、丙二醛(MDA)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)及环磷酸腺苷(cAMP)发生显著改变(P<0.05)。与模型组相比,SQBQ和单味中药均能不同程度改善心衰大鼠心脏功能,抑制心肌纤维化,减轻心肌损伤及氧化损伤。代谢组学结果显示,与假手术组相比,模型组筛选出Oxidized glutathione、Glucosylsphingosine等55个差异代谢物,益气中药对其中Nonadecanoic acid、Tetradecanoylcarnitine等41个代谢物有干扰(P<0.05),其中SQBQ影响34个,人参影响25个,黄芪影响28个,共同扰动的代谢物有21个,涉及的代谢通路有能量代谢、嘌呤代谢、谷胱甘肽代谢、肉碱代谢和氨基酸代谢等,SQBQ能够特异性调节脂质代谢。结论SQBQ能够改善心衰大鼠心脏功能,减轻心肌损伤,可能与其能够调节脂质代谢、氨基酸代谢、能量代谢、谷胱甘肽代谢等途径有关,且SQBQ整体抗心衰作用优于单味人参和黄芪,体现了中药复方的配伍协同优势。
Objective To investigate the therapeutic effect of Shenqi Buqi granule(SQBQ)on rats with chronic heart failure and the rationality of its compatibility.Methods SPF-grade male SD rats were randomly divided into sham group,model group,SQBQ group(6.93 g/kg),Ginseng group(0.63 g/kg),Astragalus group(6.30 g/kg),and Fosinopril sodium group(1.05 mg/kg).A post-myocardial infarction(MI)heart failure model was established in rats.The rats were given intragastric administration continuously for 28 days.Ultrasound was used to detect cardiac function and structure,pathological changes of myocardial tissue were observed,hematological indicators such as myocardial enzymes,heart failure markers,and inflammatory factors were measured,and metabolomic profiling of myocardial tissue was performed.Results Compared with the sham group,the model group showed significant changes in ventricular structure,systolic and diastolic dysfunction,and a remarkable decrease in cardiac function(P<0.01).Myocardial cell edema and necrosis,extensive infiltration of inflammatory cells,and obvious fibrosis were observed in model group.Serum levels of CK,CK-MB,cTnT,and NT-proBNP were significantly increased(P<0.05),plasma levels of SOD,MDA,IL-6,TNF-α,and cAMP showed significant changes(P<0.05)in model group.Compared with the model group,both SQBQ and single Chinese herbal medicines improved cardiac function,inhibited myocardial fibrosis,alleviated myocardial injury and oxidative damage in heart failure rats.Metabolomic analysis showed that 55 differential metabolites such as Oxidized glutathione,Glucosylsphingosine were identified in the model group compared with the sham group.Chinese herbs with Qi-invigorating effect interfered with 41 metabolites including nonadecanoic acid and tetradecanoylcarnitine(P<0.05),among which 34 were affected by SQBQ,25 by ginseng,28 by astragalus.There were 21 metabolites commonly disturbed by the three.The involved metabolic pathways included energy metabolism,purine metabolism,glutathione metabolism,carnitine metabolism,and amino acid metabolism.Notably,SQBQ could specifically regulate lipid metabolism.Conclusion SQBQ can improve cardiac function and reduce myocardial injury in heart failure rats,which may be related to its regulation of metabolic pathways such as lipid metabolism,amino acid metabolism,energy metabolism,and glutathione metabolism.Moreover,the overall anti-heart failure effect of SQBQ is superior to that of single ginseng or astragalus,demonstrating the synergistic advantage of Chinese herbal compound prescriptions.
作者
苗兰
林力
孟硕
彭勍
任常英
孙明谦
刘建勋
MIAO Lan;LIN Li;MENG Shuo;PENG Qing;REN Changying;SUN Mingqian;LIU Jianxun(Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China)
出处
《中国中医基础医学杂志》
2026年第2期291-299,共9页
JOURNAL OF BASIC CHINESE MEDICINE
基金
国家自然科学基金面上项目(82274141)
中国中医科学院西苑医院能力提升项目(XYZX0405-16)
国家中医心血管病临床医学研究中心专项科研基金(CMC2022007)。
关键词
参芪补气颗粒
慢性心力衰竭
代谢组学
配伍作用
Shenqi Buqi granule
Chronic heart failure
Metabolomics
Compatibility effect
