摘要
探讨miRNA-21-3p在急性髓系白血病(AML)患者外周血单个核细胞(PBMC)中的表达水平变化,并分析其临床意义。方法:采用qRT-PCR法测定正常对照和AML患者PBMC的miRNA-21-3p表达水平,根据ROC曲线计算miRNA-21-3p临界值(cut-off值)。体外CCK8细胞计数法测定白血病细胞的增殖水平。分别比较不同患者组的miRNA-21-3p表达水平,并根据cut-off值分为大于和小于两组,同时分析其临床预后意义。结果:①急性髓系白血病(AML)患者与正常对照组比较,PBMCmiRNA-21-3p平均表达水平高于正常对照组,其临界cut-of值为1.426^(2^-△△Ct)。②不同组白血病患者miRNA-21-3p水平的比较:未复发组AML患者的PBMC O miRNA-21-3p水平明显低于复发组(P=0.0027),完全缓解(CR)组与未缓解(NR)组的PBMCmiR-21-3p水平差别不明显(t=1.254,P=0.2146),MRD(+)组PBMCmiRNA-21-3p水平高于MRD(-)组(P=0.0001)。③大于和小于临界值两组的临床意义分析:PBMCmiRNA-21-3p小于临界值组的无病生存期(DFS)与大于临界值组无明显差异(P=0.2331),大于临界值组的复发率和微小残留病(MRD)阳性率均高于小于临界值组(P<0.0001)。大于临界值组和低于临界值组相比较,完全缓解率无明显差异,但是PBMCmiRNA-21-3p大于临界值组的5年生存率低于小于临界值组(P=0.0109)。④CCK8细胞计数法测定miRNA-21-3pmimics对白血病细胞增殖的影响。结果表明miR-21-3pmimics对U937、THP1和K562细胞的增殖水平并没有明显的直接作用(P>0.05)。结论:PBMCmiRNA-21-3p水平与白血病预后有关,并以大于和小于临界值分组更为敏感。miRNA-21-3p mimics对U937、THP1和K562细胞的增殖水平没有明显的直接作用。
Objective:To detect the expression level of miRNA-213p in peripheral blood mononuclear cells(PBMC)of patients with acute myoid leukemia(AML)and to investigate its clinical significance.Methods:The expression level of miRNA-213p in peripheral blood mononuclear cells(PBMC)was determined by qRTPCR and the critical value(cut-off value)was calculated according to the ROC curve.The CCK8 cell counting method was used to determine the level of leukemia proliferation.According to the cut-off value,the patients were divided into two groups:greater than and less than,and the clinical prognostic significance of each group was analyzed.Results:①The average expression level of PBMC miRNA-21-3p in patients with AML was higher than that of the control group,and the critical cut-off value was 1.426^(2^-△△Ct).②Comparison of miRNA213p in patients with leukemia in different groups:The results of the detection showed that the level of PBMC miRNA-21-3p in patients with AML in non-relapse group was significantly lower than that in the relapse group(P=0.0027),and there was no significant difference in the level of PBMC miRNA−21−3p between the complete remission(CR)group and the non−remission(NR)group(t=1.254,P=0.2146),and the level of PBMC miRNA−21−3p in the MRD(+)group was higher than that in MRD()group(P=0.0001).③Clinical significance analysis of the two groups greater than and less than the critical value:The disease-free survival(DFS)of the PBMC miRNA-213p less than the critical value group was not significantly different from that of the greater than the critical value group(P=0.231),the recurrence rate and the positive rate of MRD were higher than that of the less than the critical value group(p<0.001).There was no significant difference in the complete remission rate between the group greater than the critical value and the group less than the critical value,but the-year survival rate of the PBMC miRNA-213p greater than the critical value group was lower than that of the less than the critical value group(P=0.0109).④CCK8 cell counting method to determine the effect of miR-21-3p mimics on the proliferation ofukemia cells.The results showed that miRNA-21-3p mimics had no significant direct effect on the proliferation level of U937,THP1 K562 cells(P>0.05).Conclusion:PBMC miRNA-213p levels are associated with prognosis of leukemia and are more sensitive to grouping greater than and less than critical value.miR-21-3p mimics has no obvious direct effect on the proliferation level of U937,THP1 and K562.
作者
吕欣颖
韦若颍
傅甜
吴沄桦
毕可红
姜国胜
LV Xin-ying;WEI Ruo-ying;FU Tian(Department of Immunology,College of Basic Medicine,Binzhou Medical University,Shandong Yantai 264003)
出处
《医学检验与临床》
2026年第1期37-41,共5页
Medical Laboratory Science and Clinics
