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油樟油及其主要成分的抗氧化与抑菌活性对比研究

Comparative Study on Antioxidant and Antimicrobial Activities of Camphor Essential Oil and Its Main Components
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摘要 对比研究了油樟油及其主要成分1,8-桉叶素、α-松油醇、β-水芹烯、香桧烯的抗氧化活性及抑菌活性。研究结果表明:油樟油及其4种成分的抗氧化活性和抑菌活性存在较大差异。当质量浓度为10 g/L时,香桧烯、油樟油、α-松油醇、1,8-桉叶素和β-水芹烯对1,1-二苯基-2-三硝基苯肼自由基(DPPH·)清除率分别为17.59%、14.40%、8.71%、8.05%和2.05%,对2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐自由基(·ABTS^(+))清除率分别为12.12%、10.34%、6.78%、6.02%和2.07%。α-松油醇对大肠杆菌、沙门氏菌及金黄色葡萄球菌的抑菌圈直径分别为15.8、17.5和27.8 mm,而1,8-桉叶素和油樟油对这3种菌的抑菌圈直径在7~9 mm之间,β-水芹烯对3种菌均无抑菌圈。α-松油醇对大肠杆菌、沙门氏菌的最低抑菌质量浓度(MIC)和最低杀菌质量浓度(MBC)值均为1.25 g/L,对金黄色葡萄球菌的MIC和MBC值分别为0.156和1.25 g/L;1,8-桉叶素对大肠杆菌、沙门氏菌的MIC值均为2.5 g/L,MBC值均为5.0 g/L。α-松油醇、1,8-桉叶素的抑菌和杀菌活性强于油樟油,而香桧烯和β-水芹烯基本无抑菌和杀菌活性。 The comparative study was conducted to evaluate the antioxidant and antibacterial activities of Camphor essential oil and its main components:1,8-cineole,α-terpineol,β-phellandrene,and sabinene.The results showed significant differences in the antioxidant and antibacterial activities between camphor essential oil and its four main components.When the mass concentration was 10 g/L,the 1,1-diphenyl-2-picrylhydrazyl radical(DPPH·)scavenging rates of sabinene,camphor essential oil,α-terpinol,1,8-cineole andβ-phellandrene were 17.59%,14.40%,8.71%,8.05%,and 2.05%,respectively.In addition,their 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)radical(·ABTS^(+))scavenging rates were 12.12%,10.34%,6.78%,6.02%,and 2.07%,respectively.The inhibition zone diameter ofα-terpinol against Escherichia coli,Salmonella and Staphylococcus aureus was 15.8,17.5,and 27.8 mm,respectively.The antimicrobial diameter of 1,8-cineole and camphor oil against the three tested bacteria was between 7 and 9 mm,andβ-phellandrene showed no inhibitory effect(no detectable zone of inhibition).The minimum inhibitory mass concentration(MIC)and minimum bactericidal mass concentration(MBC)values ofα-terpineol against E.coli and Salmonella were both determined to be 1.25 g/L.In contrast,the MIC and MBC values for S.aureus were found to be 0.156 g/L and 1.25 g/L,respectively.For 1,8-cineole,the MIC values against E.coli and Salmonella were both established at 2.5 g/L,while the corresponding MBC values were recorded as 5.0 g/L for both bacterial species.α-Terpineol and 1,8-cineole demonstrated significantly stronger antibacterial and bactericidal activity compared to camphor essential oil,while sabinene andβ-phellandrene showed negligible antimicrobial activity.
作者 车丽涛 任永军 武启繁 杨雅茹 杨晓杰 明洁 CHE Litao;REN Yongjun;WU Qifan;YANG Yaru;YANG Xiaojie;MING Jie(Faculty of Agriculture,Forestry and Food Engineering,Sichuan Province Engineering Technology Research Center of Oil Cinnamon,Yibin University,Yibin 644000,China;Sichuan Animal Science Academy,Animal Breeding and Genetics Key Laboratory of Sichuan Province,Chengdu 610066,China)
出处 《林产化学与工业》 北大核心 2026年第1期153-159,共7页 Chemistry and Industry of Forest Products
基金 四川省科技计划资助项目(2023YFN0076) 宜宾学院高层次人才“启航”计划项目(2021QH031) 四川省油樟工程技术研究中心开放基金项目(22YZZ08)。
关键词 油樟油 抗氧化 抑菌 Α-松油醇 1 8-桉叶素 camphor essential oil antioxidant activity antibacterial activity α-terpineol 1,8-cineole
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