摘要
【目的】本研究以阿勒泰羊为研究对象,对SMAD家族成员1(SMAD1)和肿瘤转移抑制基因(KISS1)与基因多态性与产羔数的相关性分析,同时利用qPCR法研究对该基因在单、双羔组的阿勒泰羊的不同组织间内mRNA差异表达量,以寻找绵羊产羔性状分子标记和绵羊育种提供科学依据。【方法】对KISS1和SMAD1基因SNP进行KASP基因分型,并将不同基因型与产羔数进行关联分析。利用qPCR法对KISS1和SMAD1基因在单、多羔阿勒泰羊9个不同组织中的相对表达水平进行检测。【结果】SMAD1基因(g.12487190G>T)2种基因型频率为0.93(GG)和0.07(GT),KISS1基因(g.1311578G>T)2种基因型频率为0.88(GG)和0.12(GT);群体遗传学分析表明,SMAD1和KISS1基因处于低度多态位点(PIC<0.25);χ^(2)检验结果表明均处于哈代温伯格(Hardy-weinberg)平衡状态(P>0.05);关联分析表明,SMAD1基因g.12487190G>T位点的GG基因型个体产羔数与GT基因型个体产羔数差异不显著(P>0.05),KISS1基因g.1311578G>T位点的GT基因型个体产羔数显著高于GG基因型个体产羔数(P<0.05);qPCR结果显示:KISS1基因主要在阿勒泰羊的子宫、垂体和下丘脑组织中表达,对不同产羔数相同组织的表达量分析表明,在多羔组中子宫组织中mRNA的表达量显著高于单羔组(P<0.05)。SMAD1基因主要在阿勒泰羊的下丘脑、子宫和垂体组织中表达,对不同产羔数相同组织表达量的分析表明,在多羔组下丘脑的mRNA的表达量显著高于单羔组(P<0.05)。【结论】KISS1基因可能与阿勒泰羊的胚胎发育密切相关,其g.1311578G>T位点可作为阿勒泰羊产羔性状选育的潜在分子标记。
【Objective】This study takes Altay sheep as the research object.Firstly,it analyzes the polymorphism of SMAD family member 1(SMAD1)and tumor metastasis suppressor gene(KISS1),as well as their correlation with litter size.Meanwhile,by means of qPCR,it investigates the differential mRNA expression levels of these two genes in different tissues of Altay sheep from the single-lamb group and twinlamb group.The purpose of this study is to identify molecular markers for sheep lambing traits and provide a scientific basis for sheep breeding.【Methods】KISS1 and SMAD1 SNP were genotyped by KASP and the correlation between different genotypes and lambing number was analyzed.The relative expression levels of KISS1 and SMAD1 genes in 9 different tissues of single and double lambs Altay sheep were detected by qPCR.【Results】The results showed that the frequencies of the two genotypes of SMAD1 gene(g.12487190G>T),were 0.93(GG)and 0.07(GT),the frequencies of the two genotypes of KISS1 gene(g.1311578G>T)were 0.88(GG)and 0.12(GT);Population genetic analysis showed that SMAD1 and KISS1 genes were low polymorphic loci(PIC<0.25);χ^(2) test results showed that the two gene loci were in Hardyweinberg equilibrium in Altai sheep(P>0.05);Association analysis showed that in SMAD1 gene g.12487190G>T,there was no significant difference in the number of lambs born between GG genotype and GT genotype at T locus(P>0.05).In KISS1 gene g.1311578G>T,the number of lambs of GT genotype at T locus was significantly higher than that of GG genotype(P<0.05);qPCR results showed that KISS1 gene expression was higher in cerebellum,spleen and hypothalamus,but lower in liver and brain of Altay sheep.In a single lamb,the expression of this gene was higher in uterus,liver and pituitary tissues,but lower in hypothalamus,brain and cerebellum tissues.The mRNA expression levels of cerebellum,uterus and heart in the two lambs group were significantly higher than those in the one lamb group(P<0.05).The expression of SMAD1 gene was higher in hypothalamus and pituitary tissues,but lower in uterus and brain tissues in Altay sheep.In single lamb,the expression levels were higher in heart,uterus and spleen,but lower in hypothalamus and brain.The mRNA expression levels of the same tissues with different lamb numbers showed that the mRNA expression levels of cerebellum and hypothalamus in the double lamb group were significantly higher than those in the single lamb group(P<0.05).【Conclusion】KISS1 gene is closely related to the embryonic development of Altay sheep,whose T locus of g.1311578G>T can be used as a potential molecular marker for the breeding of lamb-producing traits in the sheep.
作者
马应国
於建国
艾布什·努尔兰
叶尔肯·黑扎提
郝耿
刘玲玲
MA Yingguo;YU Jianguo;Aibushi Nuerlan;Yeerken Heizati;HAO Geng;LIU Lingling(Institute of Animal Sciences,Xinjiang Uygar Autonomous Region Academy of Animal Science,Urumqi 830000,China;College of Animal Science,Xinjiang Agricultural University,Urumqi 830000,China;Gannan Prefecture Academy of Agriculture,Forestry and Grassland Sciences,Gansu Province,Hezuo Gansu 747000,China;Agricultural Service Development Center,Qiganjidi Township,Fuhai County,Fuhai Xinjiang 836400,China)
出处
《新疆农业科学》
北大核心
2025年第9期2276-2285,共10页
Xinjiang Agricultural Sciences
基金
国家肉羊产业技术体系(CARS-38)
国家重点研发计划项目(2021YFD1600702)。
