摘要
目的建立高尿酸血症大鼠模型,研究茯苓、车前子及茯苓-车前子配伍降血尿酸的作用及机制。方法将54只雄性SPF级SD大鼠随机分为空白组、模型组、别嘌醇组、茯苓组、车前子组、茯苓-车前子配伍组,采用灌胃1000 mg/(kg·d)氧嗪酸钾造模药建立高尿酸血症大鼠模型,以别嘌醇为阳性对照,观察车前子、茯苓及茯苓-车前子配伍对大鼠外观状态、体质量的影响,利用酶联免疫法测定血清尿酸(SUA)、血尿素氮(BUN)、血肌酐(Scr)、尿酸盐转运体(UAT)、有机阴离子转运体(OAT)、肝组织黄嘌呤氧化酶(XOD)、肝组织腺苷脱氨酶(ADA)水平,利用蛋白免疫印迹法测定肾脏组织OAT1、尿酸盐阴离子转运体1(URAT1)蛋白表达。结果(1)与空白组相比,模型组体质量无显著性变化(P>0.05),模型组SUA含量升高(P<0.05),模型组肝组织XOD、ADA的含量升高(P<0.05),血清UAT、OAT含量降低(P<0.05),模型组肾脏组织URAT1蛋白表达量升高(P<0.05)、OAT1蛋白表达量降低(P<0.05)。(2)与模型组相比,车前子组、茯苓组及茯苓-车前子配伍组SUA的含量降低(P<0.05),肝组织XOD、ADA的含量降低(P<0.05),血清UAT、OAT的含量升高(P<0.05),肾脏组织URAT1蛋白表达量降低(P<0.05)、OAT1蛋白表达量升高(P<0.05)。结论连续4周灌胃1000 mg/kg氧嗪酸钾可成功建立高尿酸血症大鼠模型,茯苓组、车前子组及茯苓-车前子配伍组均可有效降低高尿酸血症大鼠XOD、ADA酶活性,抑制尿酸的生成,其可能通过利水渗湿祛除体内湿邪,促进水液代谢,增加OAT1蛋白水平,促进尿酸排泄,达到降尿酸的目的。
Objective To establish a hyperuricemia rat model and investigate the effects and mechanisms of Fuling(Poria),Cheqianzi(Plantaginis Semen),and the Fuling(Poria)-Cheqianzi(Plantaginis Semen)combination in reducing blood uric acid.Methods Fifty-four male SPF-grade SD rats were randomly divided into the control group,model group,allopurinol group,Fuling group,Cheqianzi group,and Fuling-Cheqianzi combination group.The rats were induced to develop hyperuricemia by oral administration of 1000 mg/(kg·d)potassium oxalate.Allopurinol was used as the positive control.The effects of Cheqianzi(Plantaginis Semen),Fuling(Poria),and the Fuling(Poria)-Cheqianzi(Plantaginis Semen)combination on the rats'appearance and body weight were observed.Serum uric acid(SUA),blood urea nitrogen(BUN),serum creatinine(Scr),urate transporter(UAT),organic anion transporter(OAT),hepatic xanthine oxidase(XOD),and hepatic adenosine deaminase(ADA)were measured using enzyme-linked immunosorbent assay(ELISA).Protein immunoblotting was employed to determine the protein expression of organic anion transporter(OAT1)and urate anion transporter 1(URAT1)in renal tissue.Results(1)Compared with the blank group,the model group showed no significant change in body weight(P>0.05),but exhibited elevated serum SUA levels(P<0.05),increased hepatic XOD and ADA content(P<0.05),decreased UAT and OAT levels(P<0.05),elevated URAT1 protein expression in ileal tissue(P<0.05),and reduced OAT1 protein expression(P<0.05).(2)Compared with the model group,the Fuling group,Cheqianzi group and Fuling-Cheqianzi combination group demonstrated decreased serum SUA levels(P<0.05)and reduced hepatic XOD and ADA content(P<0.05);increased UAT and OAT levels(P<0.05),decreased URAT1 protein expression in ileal tissue(P<0.05),and increased OAT1 protein expression(P<0.05).Conclusion The rat model of hyperuricemia was successfully established by continuous intragastric administration of 1000 mg/kg potassium oxalate for 4 weeks.Both Fuling group,Cheqianzi group and Fuling-Cheqianzi combination group could effectively reduce the XOD and ADA enzyme activities in hyperuricemic rats,inhibit the production of uric acid,which may be achieved by promoting diuresis and eliminating dampness to remove dampness from the body,promoting water metabolism,increasing the level of OAT1 protein,and promoting the excretion of uric acid.
作者
曹亚茹
伍小芳
赵星宇
张奇
李林泽
张凤伟
蒋明慧
田昕
CAO Yaru;WU Xiaofang;ZHAO Xingyu;ZHANG Qi;LI Linze;ZHANG Fengwei;JIANG Minghui;TIAN Xin(Beijing University of Chinese Medicine,Beijing 102488,China)
出处
《辽宁中医药大学学报》
2026年第2期46-50,F0003,共6页
Journal of Liaoning University of Traditional Chinese Medicine
基金
国家重点研发计划中医药现代化研究重点专项(2018YFC1706800)。
关键词
高尿酸血症
茯苓
车前子
配伍
降尿酸
作用机制
hyperuricemia
Fuling(Poria)
Cheqianzi(Plantaginis Semen)
compatibility
uric acid reduction
mechanism of action
