摘要
化学蛋白组学是一种广泛应用于中药活性成分发现及靶点鉴定的技术,可系统阐明中药的作用机制。其核心是通过设计特异性分子探针,精准识别中药活性成分的靶点蛋白,为揭示药效物质基础及新药研发提供理论支持。我国中草药资源丰富,但中药的具体作用机制及“科学性”曾饱受质疑与批评。化学蛋白组学凭借其高特异性与系统性,已成为推动中医药现代化研究的关键工具。本文综述了近五年基于化学蛋白组学鉴定中药活性成分靶点的研究,从标记法和非标记法两个角度进行剖析,分析不同鉴定方法的优势与不足,以期为中药活性成分的靶点鉴定研究提供方法借鉴,助力中医药研究现代化进程。
Chemical proteomics is a technology widely used in the discovery of active ingredients in traditional Chinese medicine(TCM)and the identification of their targets,enabling the systematic elucidation of TCM's mechanisms of action.Its core lies in designing specific molecular probes to accurately identify target proteins of active TCM components,providing a theoretical basis for revealing pharmacodynamic material foundations and supporting new drug development.China is rich in herbal medicine resources,yet the specific mechanisms and"scientific validity"of TCM have long faced skepticism and criticism.Due to its high specificity and systematic approach,chemical proteomics has become a key tool in promoting the modernization of TCM research.This review summarizes studies from the past five years on identifying targets of active TCM ingredients using chemical proteomics,analyzing both label-based and label-free methods,and discusses the advantages and limitations of different identification strategies.The aim is to provide methodological references for target identification of active TCM components and contribute to the modernization of TCM research.
作者
曹婷
林琦越
何伟仪
何缙霖
陈梓铧
张倩
王继刚
罗飘
CAO Ting;LIN Qi-yue;HE Wei-yi;HE Jin-lin;CHEN Zi-hua;ZHANG Qian;WANG Ji-gang;LUO Piao(Guangdong Basic Research Center of Excellence for Integrated Traditional and Western Medicine for Qingzhi Diseases,Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics,School of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China;Artemisinin Research Center,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)
出处
《药学学报》
北大核心
2026年第1期47-57,共11页
Acta Pharmaceutica Sinica
基金
国家自然科学基金资助项目(82304827)。
关键词
化学蛋白组学
中药活性成分
靶点鉴定
分子探针
作用机制
chemical proteomics
active ingredients of traditional Chinese medicine
target identification
molecular probe
mechanisms of action
