摘要
Background Enterobacterial translocation is a leading contributor to fatal infection among patients with acute ischaemic stroke(AIS).Accumulative evidence suggests that mesenchymal stem cell(MSC)effectively ameliorates stroke outcomes.Whether MSC could inhibit post-stroke enterobacterial translocation remains elusive.Methods Patients with AIS and healthy individuals were enrolled in the study.Mice subjected to transient middle cerebral artery occlusion were treated with bone marrow-derived MSC(BM-MSC)right after reperfusion.Enterobacterial translocation was evaluated with Stroke Dysbiosis Index and circulating endotoxin.Thickness of mucus was assessed with Alcian blue staining.Hepatic glucocorticoid(GC)metabolism was analysed with expression of HSD11B2,HSD11B1 and SRD5A1.Results We report that the gut mucus layer was attenuated after the stroke leading to pronounced enterobacterial translocation.The attenuation of the gut mucus was attributed to diminished mucin production by goblet cells in response to the elevated systemic GC after cerebral ischaemia.Transferred-BM MSC restored the mucus thickness,thus preserving gut microbiota homeostasis and preventing enterobacterial invasion.Mechanistically,the transferred-BM MSC stationed in the liver and enhanced peroxisome proliferator-activated receptorγsignalling in hepatocytes.Consequently,expression of HSD11B2 and SRD5A1 was increased while HSD11B1 expression was downregulated which promoted GC catabolism and subsequently restored mucin production.Conclusions Our findings reveal that MSC transfer improves post-stroke gut barrier integrity and inhibits enterobacterial translocation by enhancing the hepatic GC metabolism thus representing a protective modulator of the liver-gut brain axis in AIS.
基金
supported by Guangzhou Key Research Program on Brain Science(202206060001 to ZL)
Guangdong Basic and Applied Basic Research Foundation(2020A1515010056 to BZ)
National Natural Science Foundation of China(82171307 to ZL)
National Natural Science Foundation of China(82271348 to WC)
Fundamental Research Funds for the Central Universities(23ykbj006 to WC)
Science and Technology Program of Guangzhou(2023B01J1002 to ZL).
