摘要
【目的】本研究旨在探究S-烯虫酯对锈赤扁谷盗Cryptolestes ferrugineus雌成虫卵巢发育以及卵黄原蛋白(vitellogenin,Vg)和卵黄原蛋白受体(vitellogenin receptor,VgR)基因表达的影响。【方法】利用RT-qPCR检测CfVg和CfVgR在锈赤扁谷盗不同发育阶段(卵、幼虫、蛹和成虫)及雌成虫不同组织(头、胸、中肠和卵巢)中的表达量。采用局部点滴法用S-烯虫酯处理锈赤扁谷盗3日龄雌成虫1,3,5和7 d时解剖卵巢,观察卵巢变化情况,观测卵巢管的长度和宽度,并利用RT-qPCR检测S-烯虫酯处理后8,16和24 h时雌成虫及处理后24 h时其卵巢中CfVg和CfVgR的表达量。【结果】CfVg和CfVgR在锈赤扁谷盗卵期微弱表达,幼虫期表达量极低,进入蛹期后表达量迅速上升,在成虫期的表达量显著升高;CfVg和CfVgR在锈赤扁谷盗雌成虫卵巢中特异性高表达,在头和胸等其他组织中微表达;S-烯虫酯处理锈赤扁谷盗后,卵巢发育速度明显快于无水乙醇处理的对照组的,卵巢管的长度和宽度随着处理时间的延长而增大,在处理后5 d时卵巢管长度显著大于对照组的,但处理后7 d时卵巢管长度与对照组的无显著差异;S-烯虫酯处理锈赤扁谷盗雌成虫后8,16和24 h时CfVg和CfVgR的表达量随着处理时间的增加而持续升高;处理后24 h时锈赤扁谷盗雌成虫卵巢中CfVg和CfVgR的表达量与对照组比显著上调。【结论】S-烯虫酯可促进锈赤扁谷盗成虫卵巢发育,上调雌成虫卵巢中CfVg和CfVgR的表达量,因此CfVg和CfVgR在S-烯虫酯调控锈赤扁谷盗卵巢发育及卵黄形成过程中可能发挥了重要作用。
【Aim】This study aims to clarify the effects of S-methoprene on the ovarian development and expression of vitellogenin(Vg)and vitellogenin receptor(VgR)genes in female adults of Cryptolestes ferrugineus.【Methods】The expression levels of CfVg and CfVgR in different developmental stages(egg,larva,pupa and adult)and different tissues(head,thorax,midgut and ovary)of female adults of C.ferrugineus were detected by RT-qPCR.The ovaries of C.ferrugineus were dissected at 1,3,5 and 7 d after exposure of the 3-day-old female adults to S-methoprene by topical drip method,and the changes of ovaries were observed.The length and width of ovarioles were observed and measured.RT-qPCR was used to detect the expression levels of CfVg and CfVgR in female adults at 8,16 and 24 h after S-methoprene treatment and in their ovaries at 24 h after S-methoprene treatment.【Results】The expression levels of CfVg and CfVgR were weak in the egg stage,extremely low in the larval stage,increased rapidly after entering the pupal stage,and increased significantly in the adult stage of C.ferrugineus.CfVg and CfVgR were specifically highly expressed in the ovaries of female adults of C.ferrugineus,but lightly expressed in the other tissues such as the head and thorax of female adults of C.ferrugineus.After S-methoprene treatment,the ovarian development speed was significantly faster than that of the control group treated with anhydrous ethanol,and the length and width of ovarioles of C.ferrugineus increased with the treatment time.The length of ovarioles of C.ferrugineus at 5 d after S-methoprene treatment was significantly greater than that of the control group,but there was no significant difference in the length of ovarioles between treatment group and the control group at 7 d after S-methoprene treatment.The expression levels of CfVg and CfVgR in female adults of C.ferrugineus increased continuously with the extension of treatment time at 8,16 and 24 h after S-methoprene treatment.Additionally,the expression levels of CfVg and CfVgR in the ovaries of C.ferrugineus at 24 h after S-methoprene treatment were significantly upregulated,as compared to those in the control group.【Conclusion】S-Methoprene can promote the ovarian development of C.ferrugineus adults and upregulate the expression levels of CfVg and CfVgR in the ovaries of female adults.Thus,CfVg and CfVgR may play an important role in S-methoprene regulating the ovarian development and vitellogenesis of C.ferrugineus.
作者
谢冰冰
包晗
宋丽雯
李燕羽
XIE Bing-Bing;BAO Han;SONG Li-Wen;LI Yan-Yu(College of Plant Protection,Gansu Agricultural University,Lanzhou 730070,China;Academy of National Food and Strategic Reserves Administration,Beijing 100037,China)
出处
《昆虫学报》
北大核心
2025年第12期1639-1648,共10页
Acta Entomologica Sinica
基金
国家重点研发计划项目(2023YFD1701200)。
