摘要
为探究嫁接技术对缓解番茄干旱胁迫的作用,以樱桃番茄‘京番串红1号’为接穗,分别以‘托鲁巴姆’‘茄砧3号’‘番砧2号’以及‘京番串红1号’自身为砧木,通过自然干旱处理,系统研究不同嫁接组合在干旱胁迫下的生长表现、光合特性、渗透调节能力、抗氧化体系及相关基因转录。结果表明:1)干旱胁迫显著抑制番茄幼苗生长,降低净光合速率(Pn)、气孔导度(Gs),并诱导膜脂过氧化和活性氧(ROS)积累;2)砧木种类显著影响嫁接植株的耐旱性,‘托鲁巴姆’砧木嫁接植株的表现最佳,相较于正常供水条件下自嫁接植株,其叶绿素总含量和类胡萝卜素含量均降低17.9%,SOD和POD活性分别升高120%和285.6%;脯氨酸、可溶性糖和丙二醛含量分别升高58.6%、41.1%和83.3%。而‘茄砧3号’‘番砧2号’虽具一定耐旱优势,但效果弱于‘托鲁巴姆’;3)基因表达分析表明,‘托鲁巴姆’砧木嫁接植株能够更好地维持光合色素合成相关基因(GSA、PBGD、PSY2、ZDS)、水通道蛋白相关基因(PIP1.2、PIP1.5、PIP2.1、PIP2.4)的表达水平,并协同上调抗氧化酶基因(SlSOD1、SlSOD2、SlPOD42、SlPOD17)的表达,从而显著增强番茄幼苗整体抗氧化防御能力。本研究揭示了不同砧木在调控番茄耐旱性方面的生理与分子差异,为干旱地区番茄耐旱栽培的砧木筛选与高效利用提供了理论依据和实践参考。
To investigate the effect of grafting technology on alleviating drought stress in tomato,cherry tomato‘Jingfan Chuanhong No.1’was used as the scion,and‘Torubam’,‘Qiezhen 3’,‘Fanzhen 2’,and self-grafted cherry tomato were used as rootstocks.Through natural drought treatment,the growth performance,photosynthetic characteristics,osmotic adjustment capacity,antioxidant system,and related gene transcription under drought stress were systematic conducted.The results showed that:1)Drought stress significantly inhibited tomato seedling growth,reduced net photosynthetic rate(Pn)and stomatal conductance(Gs),and induced membrane lipid peroxidation and reactive oxygen species(ROS)accumulation.2)The type of rootstock significantly influenced the drought tolerance of grafted plants.The performance of‘Torubam’rootstockgrafted plantswas the best.Compared with self-grafted plants under normal water supply,the total chlorophyll content and carotenoid content of‘Torubam’-grafted plants decreased by 17.9%,while the activities of SOD and POD increased by 120%and 285.6%,respectively.The contents of proline,soluble sugar,and malondialdehyde(MDA)increased by 58.6%,41.1%,and 83.3%,respectively.Although‘Qiezhen 3’and‘Fanzhen 2’provided some drought tolerance advantages,their effects were weaker than‘Torubam’.3)Gene expression analysis indicated that grafted plants with‘Torubam’rootstock can better maintain the expression levels of genes related to photosynthetic pigment synthesis(GSA,PBGD,PSY2,ZDS)and aquaporin-related genes(PIP1.2,PIP1.5,PIP2.1,PIP2.4),while coordinately upregulating the expression of antioxidant enzyme genes(SlSOD1,SlSOD2,SlPOD42,SlPOD17),thereby significantly enhancing the overall antioxidant defense capacity of tomato seedlings.This study reveals the physiological and molecular differences among rootstocks in regulating tomato drought tolerance,providing a theoretical basis and practical reference for rootstock selection and efficient utilization in drought-prone areas for tomato cultivation.
作者
张子怡
陈婕
季延海
刘明池
梁浩
王星
ZHANG Ziyi;CHEN Jie;JI Yanhai;LIU Mingchi;LIANG Hao;WANG Xing(College of Landscape and Ecological Engineering,Hebei University of Engineering,Handan 056009,China;Institute of Vegetables,Beijing Academy of Agricultural and Forestry Sciences/Key Laboratory of North China Urban Agriculture,Ministry of Agriculture and Rural Affairs/National Engineering Research Center for Vegetables,Beijing 100097,China)
出处
《中国农业大学学报》
北大核心
2026年第1期59-72,共14页
Journal of China Agricultural University
基金
北京市农林科学院科技创新能力建设专项(KJCX20240407、KJCX20251305)
财政部和农业农村部:国家现代农业产业技术体系资助项目(CARS-23-G-06)
北京市农林科学院蔬菜研究所茄果类协同创新中心建设项目(XTCX202303)。
关键词
砧木筛选
樱桃番茄
生理机制
抗氧化防御
基因表达
rootstock screening
Cherry tomato
physiological mechanisms
antioxidant defense
gene expression
