摘要
目的:通过检测服用参桂汤后急性心肌缺血大鼠心电图及血流动力学指标,探究参桂汤干预心律失常的作用机制。方法:将84只SD大鼠随机分为空白对照组(NC组)、假手术组(Sham组)、模型组(MI组)、胺碘酮组(PC组)、参桂汤高剂量组(SHD组)、参桂汤中剂量组(SMD组)、参桂汤低剂量组(SLD组)。灌胃相应剂量药物14 d。结扎左冠状动脉后,分别记录冠状动脉结扎前(0 min)及术后10、30、60、360 min各组大鼠标Ⅱ导联心电图和J点电压值的变化。记录实验血流动力学检测左心室收缩压(LVSP)、左室舒张末压(LVEDP)、心率(HR)、左心室内压最大上升速率(+dp/dt_(max))、左心室内压最大下降速率(-dp/dt_(max))。结果:参桂汤各剂量组及PC组在结扎后10、30、60、360 min均能有效降低J点抬高值,J点抬高程度均明显低于MI组(P<0.01)。与MI组相比,SHD组于结扎后360 min降低J点抬高值更明显(P<0.01);SMD组于结扎后降低J点程度抬高值不如PC组明显(P<0.05),而结扎后10 min与PC组相比差异无统计学意义(P>0.05);SLD组于结扎后各时间点降低J点抬高值均不如PC组明显(P<0.05)。血流动力学检测结果显示,与MI组比较,参桂汤各剂量组LVEDP明显降低(P<0.05或P<0.01),LVSP、+dp/dt_(max)、-dp/dt_(max)、心率明显升高(P<0.05或P<0.01)。结论:参桂汤各剂量组均可以降低冠状动脉结扎大鼠J点抬高电压值,使其处于电稳定状态,且血流动力学检测结果显示参桂汤可显著降低LVEDP值,并能明显升高LVSP、+dp/dt_(max)、-dp/dt_(max)、心率,以高剂量参桂汤、胺碘酮效果更好。
Objective:To investigate the protective effect of Shengui Decoction on arrhythmia in rats with acute myocardial ischemia model.Methods:Eighty-four SD rats were randomly divided into the blank control group(NC group),the sham operation group(Sham group),the model group(Ml group),the amiodarone group(PC group),the Shengui Decoction high dose group(SHD group),the Shengui Decoction medium dose group(SMD group),and Shengui Decoction low dose group(SLD group).The corresponding dose of the drug was gavaged for 14 days.After ligating the left coronary artery,changes in the standard Ⅱ lead electrocardiogram and J-point voltage were recorded of the rats in each group at 0 minutes(pre-ligation)and at 10,30,60,and 360 minutes post-ligation.The left ventricular systolic pressure(LVSP),lef ventricular end-diastolic pressure(LVEDP),heart rate(HR),_(max)imum rate of increase in left ventricular pressure(+dp/dt_(max)),and _(max)imum rate of decrease in left ventricular pressure(—dp/dt_(max))were detected through Hemodynamics.Results:All dosage groups of Shengui Decoction and the PC group the elevation of the J-point at 10,30,60,and 360 minutes post-ligation effectively were reduced,and the J-point elevation significantly lower than that in the Ml group(P<0.01).Compared with the Ml group,the SHD group exhibited a more pronounced reduction in J-point elevation at 360 min post-ligation(P<0.01).The SMD group showed a less significant reduction in J-point elevation compared with that in the PC group at 360 min post-ligation(P<0.05),while the difference at 10 min post-ligation was not statistically significant with the PC group(P>0.05).At all time points post-ligation,the reduction in J-point elevation in the SLD group was less pronounced than that in the PC group(P<0.05).The results of Hemodynamic showed that compared with the MI group,LVEDP significantly in all dosage groups of Shengui Decoction decreaed(P<0.05 or P<0.01),while LVSP,+dp/dt_(max),-dp/dt_(max),and heart rate increasd(P<0.05 or P<0.01).Conclusion:All dosage groups of Shengui Decoction reduced the voltage elevation at the J-point in rats with coronary artery ligation,with stable state.Hemodynamic showed that Shengui Decoction could significantly decrease LVEDP,while significantly increased LVSP,+dp/dt_(max),—dp/dt_(max),and heart rate.The high-dose Shengui Decoction and amiodarone showed better effects.
作者
王凤儒
李凤霞
李冰菲
吴尘
李国建
周琳琳
郑亚都
徐京育
WANG Fengru;LI Fengxia;LI Bingfei;WU Chen;LI Guojian;ZHOU Linlin;ZHENG Yadu;XU Jingyu(First Affiliated Hospital,Heilongjiang University of Chinese Medicine,Harbin 150040,Heilongjiang,China;Heilongjiang University of Chinese Medicine)
出处
《中西医结合心脑血管病杂志》
2026年第3期357-364,共8页
Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
基金
黑龙江省中医药科研项目(No.ZHY2024-071)。
关键词
急性心肌缺血
心律失常
参桂汤
胺碘酮
心电图
实验研究
acute myocardial ischemia
arrhythmia
Shengui Decoction
amiodarone
electrocardiogram
experimental study
