摘要
建立食凉茶标准汤剂的质量控制方法,为其进一步开发利用提供标准化参考。从有效性和真实性出发,对性状、薄层色谱鉴别、出膏率、pH值、特征图谱、含量测定等开展研究。结果显示16批食凉茶标准汤剂的出膏率、山柰酚-3-O-芸香糖苷含量及转移率分别为30.52%~39.78%、0.61~1.73 mg/mL、49.80%~79.26%,HPLC特征图谱共标定7个共有峰,其中6个共有峰分别为芦丁、异槲皮苷、山柰酚-3-O-芸香糖苷、槲皮苷、槲皮素、山柰酚,相似度均大于0.90。食凉茶标准汤剂质量稳定、可控性好,建立的质量控制方法准确、可靠,可为该标准汤剂的质量标准建立提供参考依据。
To establish quality control methods for standard decoction of shiliang tea,and provide standardized references for its further development and utilization,from the perspective of effectiveness and authenticity,included appearance characteristics,TLC,pH value,extract rate,characteristic chromatogram and content determination,were carried out.The results showed that the extract rate,content of kaempferol-3-O-rutinoside and the transfer rate of 16 batches of standard decoction of shiliang tea were 30.52%to 39.78%,0.61 to 1.73mg/mL,49.80%to 79.26%,respectively.A total of 7 common peaks were marked in HPLC characteristic spectrum,of which 6 common peaks were rutin,isoquercitrin,kaempferol-3-O-rutinoside,quercitrin,quercetin,kaempferol,and the similarity was all greater than 0.90.The quality of standard decoction of shiliang tea was stable and controllable,and the established quality control methods were accurate and reliable,which could provide reference basis for the establishment of quality standards of this standard decoction.
作者
毛菊华
陈梦
吴查青
王发英
钱爱华
王伟影
MAO Juhua;CHEN Meng;WU Chaqing;WANG Faying;QIAN Aihua;WANG Weiying(Lishui Institute for Quality Inspection and Testing,Zhejiang Lishui 323000,China)
出处
《广州化工》
2026年第2期72-75,79,共5页
GuangZhou Chemical Industry
基金
丽水市公益性技术应用研究项目(2023SJZC053)。
关键词
食凉茶
标准汤剂
特征图谱
含量测定
Shiliang tea
standard decoction
characteristic chromatogram
content determination
