摘要
为研究消油剂对水生生物的毒性效应,以海湾扇贝(Argopecten irradians)为研究对象,通过暴露7 d与恢复8 d实验,探讨不同浓度消油剂(10、50、100μg/L)与0号柴油乳化液(0.025、0.100、0.200μg/L)对海湾扇贝肝脏超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化氢酶(Catalase,CAT)活性的影响。实验结果表明:暴露阶段消油剂三个浓度组、0号柴油乳化液两个浓度组(0.025、0.100μg/L)的海湾扇贝SOD、CAT均被诱导,诱导的时间和程度不同,表现出较强的敏感性和诱导性。浓度越高,酶活性最大值出现时间越早;高浓度组0号柴油乳化液对SOD活性呈抑制效应,对活性的抑制作用也早于低浓度组,呈现出较明显的浓度效应。释放阶段结束时,消油剂实验组中海湾扇贝SOD活性明显高于对照组,高浓度组CAT活性低于对照组,且有显著差异。0号柴油乳化液实验组中海湾扇贝SOD、CAT活性与对照组有显著差异。海湾扇贝肝脏SOD、CAT活力能够反映消油剂、0号柴油乳化液对生物体产生的毒害作用。
To study the toxicity of dispersant use upon aquatic organisms,Argopecten irradians was chosen as the research object to investigate the effects of dispersant(10,50,100μg/L)and chemically dispersed 0 diesel(0.025,0.100,0.200μg/L)on the activities of superoxide dismutase(SOD)and catalase(CAT)of the digestive gland at different concentrations by exposure(7 d)and depuration tests(8 d).Results showed that the activities of SOD and CAT were induced in three concentrations of dispersant and two concentrations of chemically dispersed 0 diesel(0.025,0.100μg/L)at exposure phase,and the induced time and degree were different under different conditions,implying that SOD and CAT had strong sensitivity and inducibility.The higher the concentration was,the earlier the activity appeared;The inhibition of SOD activity in the high concentration group of chemically dispersed 0 diesel were higher than that in the low concentration group,and the activity inhibition in the high concentration group was earlier than that in the low concentration group,showing the obvious concentration effects.At the end of depuration phase,the SOD activities in the experimental group were significantly higher than that in the control group;However,the CAT activities in the experimental group were significantly lower than that in the control group of dispersant.The SOD and CAT activities of Argopecten irradians had significant differences between the experimental group and the control group of chemically dispersed 0 diesel.And the SOD and CAT activities of Argopecten irradians’s liver could reflect the toxic effects of dispersant and chemically dispersed 0 diesel.
作者
耿仁闯
崔正国
王庆奎
李世林
徐勇
Geng Renchuang;Cui Zhengguo;Wang Qingkui;Li Shilin;Xu Yong(Tianjin Key Laboratory of Aquatic Ecology and Aquaculture,College of Fisheries,Tianjin Agricultural University,Tianjin 300392,China;State Key Laboratory of Mariculture Biobreeding and Sustainable Goods,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,Shandong Province,China;National Engineering Research Center for Marine Aquaculture,Zhejiang Ocean University,Zhoushan 316022,Zhejiang Province,China)
出处
《天津农学院学报》
2026年第1期42-47,共6页
Journal of Tianjin Agricultural University
基金
中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金(2023TD53)
国家海洋局海洋溢油鉴别与损害评估技术重点实验室开放基金资助项目(201317)。
关键词
海湾扇贝
消油剂
0号柴油乳化液
抗氧化酶
Argopecten irradians
dispersants
chemically dispersed 0 diesel
antioxidant enzyme
