摘要
探究不同分子量和单糖组成的高良姜多糖(Alpinia officinarum Hance polysaccharides,AOHP)对乙醇诱导人胃黏膜细胞GES-1细胞损伤的保护作用.通过汤剂制备法和阴离子交换柱层析DEAE-52获得4种高良姜多糖组分(AOHP1~4),采用苯酚-硫酸法、比旋光法和高效凝胶渗透色谱法、高效液相色谱法等测定其化学组成、分子量分布和单糖组成.其中AOHP1为均一酸性杂多糖,AOHP2为精制多糖,由不同摩尔比的甘露糖(Man)、鼠李糖(Rha)、葡糖醛酸(GlcA)、半乳糖醛酸(GalA)、葡萄糖(Glc)、半乳糖(Gal)、木糖(Xyl)和阿拉伯糖(Ara)组成,量均相对分子量(Mw)分别为159.25和340.41,数均相对分子量(Mn)为131.378和42.861,多分散性指数(Mw/Mn)分别为1.21和7.94.实验表明在0~2 500μg·mL^(-1)浓度范围内两者对GES-1细胞无毒性作用,且呈现剂量依赖性的保护作用,其中AOHP2效果更优;当AOHP2≥250μg·mL^(-1)时,保护作用显著增强(P<0.05).研究证实AOHP具有胃保护作用,为高良姜资源的开发利用提供了科学依据和实验基础.
This study investigated the protective effect of Alpinia officinarum Hance Polysaccharides(AOHP)with different molecular weights and monosaccharide compositions against on GES-1 cell damage induced by ethanol.4 AOHP fractions(AOHP1~4)were obtained through decoction preparation and DEAE-52 anion-exchange chromatography.Chemical composition,molecular weight distribution,and monosaccharide composition were determined using phenol-sulfuric acid method,polarimetry,high-performance gel permeation chromatography(HPGPC),and high-performance liquid chromatography(HPLC).AOHP1 was identified as a homogeneous acidic heteropolysaccharide,while AOHP2 was a refined polysaccharide fraction composed of mannose(Man),rhamnose(Rha),glucuronic acid(GlcA),galacturonic acid(GalA),glucose(Glc),galactose(Gal),xylose(Xyl),and arabinose(Ara)in varying molar ratios.The weight-average relative molecular weights(Mw)were 159.25 and 340.41 kDa,number-average relative molecular weights(Mn)were 131.378 and 42.861 kDa,and polydispersity indices(Mw/Mn)were 1.21 and 7.94,respectively.MTT assays demonstrated that both fractions showed no cytotoxicity at concentrations of 0~2500μg·mL^(-1) and exhibited dose-dependent protective effects,with AOHP2 showing superior efficacy.Significant protective effects(P<0.05)were observed when AOHP2 concentration reached≥250μg·mL^(-1).The study confirmed that AOHP had the protective effect on the gastric mucosa,which provided the scientific basis and experimental foundation for the development and utilization of Alpinia officinarum Hance resources.
作者
罗碧
黄莉
庞亚琪
黄代君
蔡满桂
黄宏杨
贤明华
马崇坚
LUO Bi;HUANG Li;PANG Yaqi;HUANG Daijun;CAI Mangui;HUANG Hongyang;XIAN Minghua;MA Congjian(School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,Guangdong,China;Guangdong Provincial Research Center on Good Agricultural Practice and Comprehensive Agricultural Development Engineering Technology of Cantonese Medicinal Materials,Guangzhou Comprehensive Experimental Station,Chinese Materia Medica Industry Technology System,Key Laboratory for Production and Development of Cantonese Medicinal Materials Under State Administration of Traditional Chinese Medicine,Guangzhou 510006,Guangdong,China,Guangzhou 510006,Guangdong,China;Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region,Shaoguan University,Shaoguan 512005,Guangdong,China)
出处
《韶关学院学报》
2025年第11期8-16,共9页
Journal of Shaoguan University
基金
广东省农村科技特派员项目(KTP20210408)
广东省粤北食药资源利用与保护重点实验室开放基金项目(FMR2023001M)。
关键词
高良姜
酸性多糖
GES-1
乙醇诱导
Alpinia officinarum Hance
acidic polysaccharide
GES-1 cell
ethanol-induced
