摘要
目的:探究镍纹样蛋白(meteorin-like protein,METRNL)基因rs4986080位点基因多态性与2型糖尿病(type 2 diabetes mellitus,T2DM)的相关性。方法:选取受试者467例,其中T2DM患者315例,正常糖耐量(normal glucose tolerance,NGT)者152例,应用聚合酶链式反应-限制性片段长度多态性(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)法检测所有研究对象METRNL基因rs4986080位点的基因多态性,分析其与T2DM的关联性。结果:T2DM组METRNL基因rs4986080位点的GG基因型构成比及G等位基因频率均高于NGT组(P<0.05)。与METRNL基因rs4986080位点为GG基因型者相比,AA+AG基因型者的腰臀比、空腹血糖(FPG)、餐后2小时血糖(2hPG)、糖化血红蛋白(HbA1c)、稳态模型评估的胰岛素抵抗指数(HOMA-IR)明显降低(P<0.05)。Logistic回归分析显示METRNL基因rs4986080位点A等位基因是T2DM发生的保护因素。结论:METRNL基因rs4986080基因多态性可能与T2DM的发生有关,A等位基因是T2DM的保护因素。
Objective:To investigate the association between the polymorphisms of meteorin-like protein(METRNL)gene rs4986080 and type 2 diabetes mellitus(T2DM).Methods:A total of 467 subjects were selected,including 315 T2DM patients and 152 individuals with normal glucose tolerance(NGT).The polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)method was used to detect the polymorphisms of METRNL gene rs4986080 in all subjects,then analyzed the association between these polymorphisms and T2DM.Results:The constituent ratio of GG genotype and the frequency of G allele at rs4986080 locus of METRNL gene was higher in the T2DM group compared to the NGT group(P<0.05).Compared to individuals with GG genotype at the METRNL gene rs4986080 locus,those with AA+AG genotypes showed significant lower waist-to-hip ratio,fasting plasma glucose(FPG),2-hour postprandial glucose(2hPG),glycated hemoglobin(HbA1c),and homeostasis model assessment of insulin resistance(HOMA-IR)(P<0.05).Logistic regression analysis showed that A allele at rs4986080 locus of METRNL gene was probably a protective factor for T2DM.Conclusion:The polymorphisms of METRNL gene rs4986080 may be associated with the occurrence of T2DM,and A allele may be a protective factor for T2DM.
作者
肖瑞峰
贾珏
吴旭楠
邓霞
王兆祥
沈聪
沈雯
赵丽
杨玲
袁国跃
XIAO Ruifeng;JIA Jue;WU Xunan;DENG Xia;WANG Zhaoxiang;SHEN Cong;SHEN Wen;ZHAO Li;YANG Ling;YUAN Guoyue(Department of Endocrinology and Metabolism,Affiliated Hospital of Jiangsu University,Zhenjiang Jiangsu 212001;Institute of Endocrine and Metabolic Diseases of Jiangsu University,Zhenjiang Jiangsu 212001;Department of Endocrinology,Affiliated Kunshan Hospital of Jiangsu University,Suzhou Jiangsu 215300,China)
出处
《江苏大学学报(医学版)》
2026年第1期58-64,共7页
Journal of Jiangsu University:Medicine Edition
基金
江苏省高层次卫生人才“六个一工程”项目(LGY2016053)
江苏省第六期“333高层次人才培养工程”(“卡脖子”技术)(BRA2022008)。
关键词
镍纹样蛋白
单核苷酸多态性
2型糖尿病
胰岛素抵抗
meteorin-like protein
single nucleotide polymorphism
type 2 diabetes mellitus
insulin resistance
