摘要
酸性蛋白酶被广泛应用于食品、动物饲料和皮革的生产等行业。其广泛存在于动物、真菌、植物、原生动物、细菌和病毒中,但曲霉属丝状真菌黑曲霉因其较高的蛋白分泌效率和安全性等原因,是目前酸性蛋白酶的主要生产者。多种不同特性的黑曲霉酸性蛋白酶已经得到了较好的解析,但依然有多种黑曲霉酸性蛋白酶未得到充分研究,为满足各行各业生产力发展的需求,还需要不断开发更高酶活、不同环境适应性的酸性蛋白酶。本文综述了黑曲霉酸性蛋白酶的分类、结构和酶学性质、影响黑曲霉分泌酸性蛋白酶的调控因素,以及黑曲霉产酸性蛋白酶的不同产酶优化策略,对未来的研究提供了借鉴和参考。
Acid proteases are extensively applied in various industries,including food,animal feed,and leather production.They are ubiquitously present in animals,fungi,plants,protozoa,bacteria,and viruses.The filamentous fungus Aspergillus niger has become the primary producer of acid proteases due to its high protein secretion efficiency and safety.Although multiple acid proteases with distinct characteristics from A.niger have been well characterized,numerous others remain insufficiently studied.In view of the growing demands for productivity across industries,it is essential to develop acid proteases with enhanced activities and wide environmental adaptability.This review summarizes the classification,structural and enzymatic properties of acid proteases produced by A.niger,regulatory factors influencing their secretion,and different optimization strategies for enhancing the acid protease production by A.niger,aiming to provide references for future research.
作者
王崇正
梅建凤
李孝辉
皮雄娥
付浩
WANG Chongzheng;MEI Jianfeng;LI Xiaohui;PI Xiong’e;FU Hao(College of Pharmaceutical Science,Zhejiang University of Technology,Hangzhou 310014,Zhejiang,China;Zhejiang Provincial Key Laboratory of Agricultural Microbiomics,Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,Zhejiang,China)
出处
《生物工程学报》
北大核心
2025年第12期4626-4649,共24页
Chinese Journal of Biotechnology
基金
浙江省重点研发计划(2024C02004)。
关键词
酸性蛋白酶
黑曲霉
蛋白酶结构
生化特性
调控因子
产酶优化
acid protease
Aspergillus niger
protease structure
biochemical properties
regulator
enzyme production optimization
