摘要
遗传转化技术在改良作物农艺性状、提高作物产量及品质等方面发挥着重要作用,而传统遗传转化方法在许多蔬菜作物生物育种中的应用受到多种因素限制。花粉磁转染法是利用磁性纳米颗粒将外源核酸直接传递至花粉中,然后通过授粉受精的方式实现外源基因的递送。该技术操作简单、无需组织培养,是极具应用前景的植物遗传转化新技术之一。本文系统介绍了花粉磁转染法的技术原理和应用优势,以及该技术在蔬菜作物遗传转化中的实际应用、存在问题等,并对其改进空间提出了展望,以期为蔬菜作物的高效生物育种提供参考。
Transgenetic technologies are crucial for the improvement of crop agronomic traits,yield,and quality.However,conventional transgenic methods face multiple limitations in biological breeding of vegetable crops.Through the method of pollen magnetofection,the exogenous nucleic acids can be directly delivered into pollen grains,and the transformation can be realized by pollination-fertilization.Due to its easy operation and tissue culture-free,pollen magnetofection is thought as one of new transformation techniques with promising application prospects.In this review,the technical principles,application advantages,practical implementation,and current challenges of pollen magnetofection in the transform of vegetable crops,are systematically introduced,and its future improvements are suggested,in order to provide a reference for efficient biological breeding in vegetable species.
作者
范海玮
雍康
王振泽
逯明辉
FAN Haiwei;YONG Kang;WANG Zhenze;LU Minghui(College of Horticulture,Northwest A&F University,Yangling 712100,Shaanxi,China)
出处
《中国蔬菜》
北大核心
2026年第1期40-48,共9页
China Vegetables
基金
国家大宗蔬菜产业技术体系专项(CARS-23-G22)
陕西省重点研发计划项目(2025NC-YBXM-043)
陕西省农业核心技术攻关项目(2024NYGG008-2)。
关键词
磁转染
花粉
遗传转化
蔬菜
生物育种
magnetofection
pollen
genetic transformation
vegetable
biological breeding
