摘要
【目的】探究枯草芽孢杆菌(Bacillus subtilis)和生物炭联合施用对山东省青岛市盐碱土改良及月季生长的影响。【方法】采集青岛市董家口港区盐碱土进行月季盆栽试验,设置4个土壤处理:施用枯草芽孢杆菌菌悬液(Bs)、2%生物炭(Bi)、枯草芽孢杆菌菌悬液+2%生物炭(Bs+Bi),以对土壤不作任何处理为对照(CK),分析不同处理对土壤理化性质、酶活性及月季生长和生理特性的影响。【结果】与CK相比,Bs+Bi处理土壤pH值、电导率显著降低4.80%、14.01%(P<0.05),土壤硝态氮、速效磷和有机质含量分别显著提高83.28%、11.68%和65.13%(P<0.05);土壤硝酸还原酶和亚硝酸还原酶活性分别显著提高7.53%和21.38%(P<0.05)。与CK相比,Bs处理月季株高与地下部生物量分别显著提高17.93%和20.10%(P<0.05),Bi处理地上部生物量显著提高29.74%(P<0.05);Bs+Bi处理叶绿素a、类胡萝卜素含量分别显著提高23.29%、79.19%(P<0.05),可溶性糖和可溶性蛋白含量分别增加13.02%和34.07%。【结论】枯草芽孢杆菌和生物炭联合施用可以有效改善盐碱土壤的理化性质、促进土壤氮循环、提高土壤酶活性,虽然对月季生长的作用效果不明显,但可以显著提高其光合色素含量并积累渗透调节物质。
【Objective】To explore the effects of the combined application of Bacillus subtilis and biochar on the improvement of saline-alkali soil and the growth of Rosa chinensis in Qingdao,Shandong Province.【Methods】A pot experiment of R.chinensis was conducted using saline-alkali soil collected from the Dongjiakou Port area in Qingdao.Four soil treatments were established:application of B.subtilis suspension(Bs),2%biochar(Bi),B.subtilis suspension+2%biochar(Bs+Bi),and untreated soil as the control(CK).The effects of different treatments on soil physicochemical properties,enzyme activities,and the growth and physiological characteristics of R.chinensis were analyzed.【Results】Compared with CK,the Bs+Bi treatment significantly reduced soil pH value and electrical conductivity by 4.80%and 14.01%,respectively(P<0.05),while significantly increasing the contents of soil nitrate nitrogen,available phosphorus,and organic matter by 83.28%,11.68%,and 65.13%,respectively(P<0.05);soil nitrate reductase and nitrite reductase activities were significantly increased by 7.53%and 21.38%,respectively(P<0.05).Compared with CK,the Bs treatment significantly increased plant height and belowground biomass of R.chinensis by 17.93%and 20.10%,respectively(P<0.05),and the Bi treatment significantly increased aboveground biomass by 29.74%(P<0.05);under the Bs+Bi treatment,chlorophyll a and carotenoid contents significantly increased by 23.29%and 79.19%(P<0.05),and soluble sugar and soluble protein contents increased by 13.02%and 34.07%,respectively.【Conclusion】The combined application of B.subtilis and biochar can effectively improve the physicochemical properties of saline-alkali soil,promote soil nitrogen cycling,and enhance soil enzyme activities.Although this combined application has no obvious effect on the R.chinensis growth,it can significantly increase the photosynthetic pigment contents and accumulate osmotic adjustment substances.
作者
王正喜
白晶
王汝法
宋京晖
尹清苓
隋龙
陈锐
张迪
WANG Zhengxi;BAI Jing;WANG Rufa;SONG Jinghui;YIN Qingling;SUI Long;CHEN Rui;ZHANG Di(Qingdao Port Construction Management Center Co.,Ltd.,Qingdao 266000,China;Qingdao Municipal Engineering Design Research Institute Co.,Ltd.,Qingdao 266061,China;Qingdao Port International Co.,Ltd.,Qingdao 266500,China;China Construction Sixth Engineering Bureau Corp.,Ltd.,Tianjin 300012,China;Nanjing Forestry University,Nanjing 210037,China)
出处
《北方农业学报》
2025年第6期77-85,共9页
Journal of Northern Agriculture
基金
国家自然科学基金项目(32471942)
国家自然科学基金青年科学基金项目(32101582)
基础研究计划(自然科学基金)——青年基金项目(BK20210613)。
关键词
枯草芽孢杆菌
生物炭
盐碱土改良
月季
生长
生理特性
Bacillus subtilis
Biochar
Saline-alkali soil improvement
Rosa chinensis
Growth
Physiological characteristics
