摘要
为研究肉苁蓉(Cistanche deserticola Y.C.Ma)大极性的化学成分及其对甲氧西林金黄色葡萄菌耐药菌(MRSA)的抑菌活性,本研究采用硅胶、MCI gel CHP-20、 ODS、 Sephadex LH-20等柱色谱和制备液相色谱等手段分离纯化肉苁蓉地下部分的70%乙醇浸提物水萃取部位,并根据理化性质和谱学数据鉴定化合物的结构,测试其抗MRSA活性。从肉苁蓉乙醇提取物水萃取部位分离和鉴定11个化合物,分别为6-去氧梓醇(1)、巴尔蒂甙(2)、鼬瓣花次甙(3)、Kankanoside B(4)、梓醇(5)、二氢梓醇(6)、益母草苷(7)、单蜜力特苷(8)、桃叶珊瑚苷(9)、Kankanoside A(10)、Kankanoside L(11),化合物5、6、8、9为首次从该植物中获得。11个环烯醚萜苷类化合物的抗MRSA活性试验结果显示化合物4、11具有潜在的抗MASR活性,其MIC50分别为56.7μmol/L和77.2μmol/L,MIC90分别为106.6μmol/L和147.6μmol/L。
This study aimed to investigate the highly polar chemical constituents of Cistanche deserticola Y.C.Ma and evaluate their antibacterial activities against methicillin-resistant Staphylococcus aureus(MRSA).Compounds were isolated through column chromatography(silica gel,MCI gel CHP-20,ODS,and Sephadex LH-20)and purified via preparative HPLC.Structural elucidation was achieved using spectroscopic analysis(MS,1 HNMR,13C NMR)and comparison with literature data.Eleven compounds were identified as 6-deoxycatalpol(1),bartsioside(2),gluroside(3),kankanoside B(4),catalpol(5),dihydrocatalpol(6),ajugol(7),monomelittoside(8),aucubin(9),kankanoside A(10),and kankanoside L(11).Compounds 5,6,8,and 9 were reported for the first time in Cistanche deserticola Y.C.Ma.Anti-MRSA evaluation revealed that compounds 4 and 11 exhibited significant inhibitory effects,with MIC50 values of 56.7μmol/L and 77.2μmol/L,and MIC90 values of 106.6μmol/L and 147.6μmol/L,respectively.
作者
江程
方晒
娄芸
吕乐
陈瑞云
JIANG Cheng;FANG Shai;LOU Yu;LYU Le;CHEN Ruiyun(School of Applied Biology,Shenzhen City Polytechnic,Shenzhen 518116,China)
出处
《海南师范大学学报(自然科学版)》
2025年第4期423-429,共7页
Journal of Hainan Normal University(Natural Science)
基金
广东省普通高校工程技术研究中心项目(2024GCZX016)
深圳城市职业学院校级课题(2411012)。
关键词
肉苁蓉
化学成分
提取分离
抗MRSA活性
Cistanche deserticola
chemical constituents
separation
anti-MRSA
