摘要
BACKGROUND Gestational diabetes mellitus(GDM)is a metabolic disorder causing hyperglycemia during pregnancy.Insulin resistance and decreased insulin secretion are linked to altered lipid metabolism that leads to progression of GDM.CD36 is a membrane glycoprotein involved in lipid metabolism and insulin sensitivity.Studies indicate that the CD36 gene is substantially linked to type 2 diabetes mellitus(T2DM)and could also influence GDM susceptibility.Insulin resistance and decreased insulin secretion are the hallmarks of T2DM,which is thought to have a similar genetic pathophysiology in GDM.AIM To investigate the impact of CD36 gene polymorphisms[rs1761667(G/A)and rs75326924(C/T)]and mRNA expression in GDM women.METHODS The case-control study involved a total of 400 pregnant women,(200 healthy controls and 200 GDM cases).The study of CD36 gene polymorphisms G/A(rs1761667)and C/T(rs75326924))were determined by polymerase chain reaction-restriction fragment length polymorphism.The mRNA expression study of CD36 gene was analyzed by quantitative polymerase chain reaction/quantitative real-time polymerase chain reaction followed by statistical analysis done using GraphPad Prism8 software(ver.8.0).RESULTS The study revealed statistically significant association(P<0.05)in anthropometric/biochemical parameters(age,gestational age,body mass index,fasting prandial glucose,post-prandial glucose,triglyceride,low-density lipoprotein)between GDM cases and healthy controls.CD36 G/A(rs1761667)and CD36 C/T(rs75326924)polymorphisms were significantly associated with GDM cases.The heterozygous genotypes(GA and CT)of both variants showed significant association(P=0.0001 and P=0.0025,odds ratio=2.683 and 2.022 respectively).Allele frequency of‘T’allele in CD36 C/T(rs75326924)polymorphism was also found to be significant(P=0.0046).CD36 gene was upregulated in individuals with GDM as compared to healthy controls(P=0.0001).However,the upregulation of gene expression was not significantly associated with the genotypes of CD36 G/A(rs1761667)and CD36 C/T(rs75326924)polymorphisms.CONCLUSION Heterozygous genotypes GA and CT of CD36 gene variants and expression are linked to GDM,potentially serving as predictive biomarkers for GDM susceptibility;further exploration needed in diverse ethnic communities.
基金
Supported by Maulana Azad National Fellowship,University Grants Commission,New Delhi,Department of Biotechnology,New Delhi,No.AS[82-27/2019(SA III)]
DBT-BUILDER-University of Lucknow Interdisciplinary Life Science Programme for Advance Research and Education(Level II),No.TG(BT/INF/22/SP47623/2022)
Department of Science and Technology-SERB Power Grant Scheme,No.SPG/2021/00545.
