摘要
为探究不同溶剂对拐枣活性成分及生物活性的影响,采用蒸馏水、不同体积分数乙醇和6种低共熔溶剂(deep eutectic solvents, DESs)提取拐枣中活性成分,并对其抗氧化活性进行探究。结果表明:蒸馏水、体积分数60%乙醇、氯化胆碱-乙二醇(摩尔比1∶2、含水量30%,DES-3)的最佳料液比为1∶30、1∶40、1∶40(g/mL),其中DES-3在料液比为1∶40(g/mL)时提取的总黄酮、总多酚、多糖、生物碱和总皂苷含量,分别达(5.21±0.04)、(22.38±0.15)、(59.27±0.85)、(6.19±0.03)、(16.53±0.14) mg/g,高于乙醇和水。抗氧化能力分析结果表明,提取物中活性成分含量与抗氧化能力呈一定量效关系,DES-3提取物对1,1-二苯基-2-三硝基苯肼(DPPH)自由基、2,2′-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)自由基清除能力和总还原力优于体积分数60%乙醇和水。
In order to investigate the effects of different solvents on the active ingredients and antioxidant activities of Hovenia dulcis,distilled water,ethanol with different volume fractions and six deep eutectic solvents(DESs)were used to extract the active components of Hovenia dulcis,and their antioxidant activity was explored.The results showed that the optimal solid-liquid ratios for distilled water,60%ethanol by volume fraction,and DES-3 were 1∶30,1∶40 and 1∶40(g/mL),respectively.Among them,choline chloride-ethylene glycol(DES-3)with a molar ratio of 1∶2 and a water content of 30%showed the highest comprehensive content of active components when the solid-liquid ratio was 1∶40(g/mL).The contents of total flavonoids,polyphenols,polysaccharides,alkaloids,and saponins reached(5.21±0.04),(22.38±0.15),(59.27±0.85),(6.19±0.03)and(16.53±0.14)mg/g,respectively,which were higher than those extracted by ethanol and water.The result of antioxidant capacity revealed a certain dose-effect relationship between the content of active ingredients in the extracts and their antioxidant capacity.The DES-3 extract exhibited superior scavenging abilities against DPPH radicals and ABTS radicals,as well as total reducing power,compared to 60%ethanol by volume fraction and aqueous extracts.
作者
侯贺丽
任双
葛伟杰
朱静
耿书宝
杨玲玲
HOU Heli;REN Shuang;GE Weijie;ZHU Jing;GENG Shubao;YANG Lingling(School of Food Sciences and Engineering,Xinyang Agriculture and Forestry University,Xinyang 464000,Henan,China)
出处
《粮食与油脂》
北大核心
2026年第1期124-131,共8页
Cereals & Oils
基金
河南省自然科学基金面上项目(242300420173)
河南省科技攻关项目(242102110090、242102110178)
河南省高等学校重点科研项目(23A550015)
河南省本科高校2023年度产教融合项目(47)
信阳农林学院科技创新团队项目(XNKJTD-001)。
关键词
拐枣
活性成分
提取溶剂
熵权法
抗氧化活性
Hovenia dulcis
active ingredient
extraction solvent
entropy weight method
antioxidant activity
