摘要
核酸等温扩增技术已被广泛应用于DNA纳米技术、数据存储及生物传感等多个领域中,对生物学领域的发展具有重要意义。因其无需热循环就在恒定温度下实现核酸扩增,更能满足现代分子检测技术快速简便的需求,为临床医学的早期诊断提供重要的应用价值。本文主要针对滚环扩增技术(rolling circle amplification,RCA)、链置换扩增技术(strand displacement amplification,SDA)、环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)、解旋酶依赖性扩增技术(helicase-dependent amplification,HDA)、重组聚合酶扩增技术(recombinase polymerase amplification,RPA)和引物交换反应(primer exchange reaction,PER)几种基于酶促反应的等温扩增技术以及杂交链式反应(hybridization chain reaction,HCR)、催化发夹组装(catalytic hairpin assembly,CHA)等非酶促等温扩增反应的技术,及其近5年在核酸检测领域的应用展开综述,聚焦目前最新进展并进行展望,以期助力等温扩增技术在该领域的迭代发展。
Nucleic acid isothermal amplification technology has been widely used in many fields such as DNA nanotechnology,data storage and biosensing,which is of great significance to the development of biology.Because it can achieve nucleic acid amplification at a constant temperature without thermal cycling,it can be adapted to different application scenarios.In addition,it can better meet the needs of modern molecular detection technology in terms of rapidity and simplicity,which can provide an important application value for early diagnosis in clinical medicine.In this article,we summarize the principles of several enzyme-based isothermal amplification techniques,including rolling circle amplification(RCA),strand displacement amplification(SDA),loop-mediated isothermal amplification(LAMP),recombinase polymerase amplification(RPA),and primer exchange reaction(PER),as well as non-enzymatic isothermal amplification techniques,such as hybridization chain reaction(HCR)and catalytic hairpin assembly(CHA),along with their applications in nucleic acid detection.This review focuses on the latest advances and future outlooks,aiming to contribute to the iterative development of isothermal amplification in this field.
作者
林梦瑶
赵爽
LIN Meng-Yao;ZHAO Shuang(School of Life Sciences,Faculty of Medicine,Tianjin University,Tianjin 300072,China)
出处
《中国生物化学与分子生物学报》
北大核心
2026年第1期54-63,共10页
Chinese Journal of Biochemistry and Molecular Biology
关键词
等温扩增
生物传感
核酸检测
isothermal amplification
biosensing
nucleic acid detection
