摘要
试验旨在建立鸡肉中芦丁含量的高效液相色谱检测方法。样品经60%乙醇(含0.01%乙酸)提取后采用C18色谱柱(4.6 mm×250.0 mm,5μm)进行分离,流动相A为甲醇,B为0.3%磷酸水,梯度洗脱程序为:0 min、40%甲醇,15 min、65%甲醇,流速0.8 mL/min,柱温30℃,检测波长360 nm,进样量20μL。结果显示,芦丁在0.2~1.4 mg/L范围内线性关系良好,标准曲线方程为y=30.674x-1.0093,相关系数(R^(2))=0.9997。加标回收试验显示,鸡肉样品的平均回收率为81.47%~90.73%,日内相对标准偏差(RSD)为2.43%~4.35%,日间RSD为5.80%~9.83%。实际样品分析显示,试验组鸡肉胸肌中芦丁含量为36.03 ng/g,腿肌中为122.32 ng/g。研究表明,试验所用方法操作简便、精密度高、回收率良好,适用于鸡肉中芦丁含量的检测。
This experiment aimed to establish an efficient liquid chromatography for detecting rutin content in chicken.Samples were extracted with 60%ethanol(containing 0.01%acetic acid)and separated using a C18 column(4.6 mm×250.0 mm,5μm).The mobile phase A was methanol and B was 0.3%phosphoric acid aqueous solution,with a gradient elution program as follows:0 min,40%methyl alcohol,15 min,65%methyl alcohol.The flow rate was 0.8 mL/min,the column temperature was 30℃,the detection wavelength was 360 nm,and the injection volume was 20μL.The results showed that rutin exhibited a good linear relationship within the concentration range of 0.2 to 1.4 mg/L,with a standard curve equation of y=30.674x-1.0093 and a correlation coefficient(R^(2))=0.9997.Spike recovery tests demonstrated average recoveries of 81.47%to 90.73%,with intra-day relative standard deviations(RSD)of 2.43%to 4.35%and interday RSD of 5.80%to 9.83%.Analysis of actual samples revealed that the rutin content in chicken breast muscle of the experimental group was 36.03 ng/g,and in leg muscle it was 122.32 ng/g.The study shows that this method is simple,precise,and provides satisfactory recovery,making it suitable for the detection of rutin content in chicken.
作者
魏瑜吁
孙云竹
刘鼎阔
李源
任超琪
赵昕
李留安
于晓雪
WEI Yuyu;SUN Yunzhu;LIU Dingkuo;LI Yuan;REN Chaoqi;ZHAO Xin;LI Liuan;YU Xiaoxue
出处
《饲料研究》
北大核心
2025年第22期137-141,共5页
Feed Research
基金
天津市青年科技人才(第三层次)项目(项目编号:QN20230301)
天津市优秀农业科技特派员项目(项目编号:24ZYCGSN00060)
天津市教委科研计划项目(项目编号:2021KJ108)。
关键词
高效液相色谱法
芦丁
鸡肉
含量测定
high-performance liquid chromatography
rutin
chicken
content determination
